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URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352
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Resultados da Pesquisa
Item Assisted selection using molecular markers linked to rust resistance SH3 gene in Coffea arabica(Crop Breeding and Applied Biotechnology, 2023-10-25) Silva, Angelita Garbossi; Ariyoshi, Caroline; Shigueoka, Luciana Harumi; Pereira, Luiz Filipe Protasio; Sera, Gustavo HiroshiThe aim of this work was to validate markers linked to the SH3 gene of coffee leaf rust (CLR) resistance and use them in assisted selection. Initially, we validated the markers in genotypes already known to carry SH3. Next, we performed phenotype and genotype evaluation for resistance to CLR in coffee plants growing under field conditions. We used Arabica coffee progenies derived from BA-10, which carries the SH3 gene due to introgression of C. liberica. Three SCAR markers (SP-M16-SH3, BA-48-21O-f, and BA-124-12K-f) and one SSR marker (Sat244) linked to SH3 gene were used to amplify the coffee plants’ DNA. Our assessments of markers validation in resistant genotypes, SP-M16-SH3 and BA-124-12K-f, were efficient to identify the SH3 gene. These two markers were used to evaluate the progenies derived from BA-10 and were significantly linked to the phenotype evaluations. The SP-M16-SH3 marker was more efficient, with the advantage of being codominant.Item Resistance to leaf rust in coffee carrying SH3 gene and others SH genes(Instituto de Tecnologia do Paraná - Tecpar, 2007-09) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Azevedo, José Alves de; Mata, João Siqueira da; Dói, Deisy Saori; Ribeiro Filho, Claudionor; Kanayama, Fabio SeidiThe aim of this work was to evaluate the resistance to rust in coffee carrying SH3 gene and other SH genes. Twenty one CIFC’s coffee trees with several resistance genes SH were evaluated in field conditions. All the evaluated coffees carrying SH3 gene presented resistance to the rust. It was possible that rust races with the virulence gene v3 in the Paraná State didn’t exist. The SH3 gene in combination with genes SH5, SH6, SH7, SH8, SH9 and SH? would be very important to obtain cultivars with more durable resistance to the rust.Item Selection for durable resistance to leaf rust using test-crosses on IAPAR-59 and Tupi IAC 1669-33 cultivars of Coffea arabica(Instituto de Tecnologia do Paraná - Tecpar, 2007-07) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Azevedo, José Alves de; Mata, João Siqueira da; Doi, Deisy Saori; Ribeiro Filho, ClaudionorThe aim of this study was to identify plants of the IAPAR-59 and Tupi IAC 1669-33 coffee cultivars with less defeated resistance genes by the rust races present at IAPAR (Londrina, Paraná State, Brazil) using test-crosses. Eighteen test-crosses derived from hybridizations between ‘IAPAR-59’ or ‘Tupi IAC 1669-33’ with susceptible coffee to the rust disease were evaluated. Six hybrids were used as susceptible standards originated from hybridizations between two susceptible coffee plants. Many parental plants of the ‘IAPAR-59’ and ‘Tupi IAC 1669- 33’ presented more defeated resistance genes against rust races present at IAPAR than others of these cultivars or the genes were in heterozygous, because of segregant susceptible plants observed in some test-crosses. The test- crosses were very efficient to identify plants with less defeated resistance genes to the H. vastatrix. Coffee plants considered resistants would must be made test-crosses to verify which plants presented less and/or more defeated genes in homozygous.Item Rust resistance in arabic coffee cultivars in northern Paraná(Instituto de Tecnologia do Paraná - Tecpar, 2013-01) Grossi, Leandro Del; Sera, Tumoru; Sera, Gustavo Hiroshi; Fonseca, Inês Cristina de Batista; Ito, Dhalton Shiguer; Shigueoka, Luciana Harumi; Andreazi, ElderThe objective of the study was to evaluate the resistance to rust in coffee cultivars developed by research institutes of Brazil in Paraná state. Resistance to the local leaf rust races was assessed in high disease intensity field conditions at Londrina and Congonhinhas in 2009 and 2010. The cultivars were developed by the EPAMIG/UFV, IAPAR, IAC and MAPA/Procafé. The resistant standard ‘IAPAR 59' and the susceptible standards Catuaí Vermelho IAC 144' and 'Bourbon Amarelo' were used. A randomized block design with three replications and plots with 10 plants was used. A scale from 1 to 5 based on the rust intensity was used to evaluate the resistance. The Catiguá MG 1, Catiguá MG 2, IAPAR 59, IPR 98, IPR 104, Palma II, Paraíso H-419-10-6-2-5-1, Paraíso H-419- 10-6-2-10-1, Paraíso H-419-10-6-2-12-1, Pau Brasil MG 1 and Sacramento MG 1 cultivars presented complete resistance to rust at Londrina and Congonhinhas. The cultivars derived from the Catucaí germplasm were susceptible or showed different levels of partial resistance. Partial resistance to the rust was observed in several coffees derived from “Hibrido de Timor”. 'Acauã' and 'Obatã IAC 1669-20' presented complete resistance at Londrina, but at Congonhinhas, they were partially resistant, indicating that different rust races have occurred at these two locations.Item Selection of Arabic coffee progenies with rust resistance(Crop Breeding and Applied Biotechnology, 2014-06) Shigueoka, Luciana Harumi; Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Mariucci Junior, ValdirThe aim of this study was to select high-yielding coffee progenies with resistance to coffee leaf rust for the State of Paraná (Brazil). Field experiments in a randomized block design were carried out in Itaguajé and Congonhinhas. Yield, vegetative vigor and rust resistance of nine progenies of Arabic coffee and three check cultivars were evaluated. Many genotypes derived from “Sarchi- mor” and “Catucaí” were susceptible. Three coffee genotypes of Sarchimor germplasm and the F 6 generation of genotype “Catuaí x (Catuaí x BA-10 coffee)” were selected to advance generations and are promising to become new cultivars for being higher-yielding than ‘IAPAR 59’ and ‘Tupi IAC 1669-33’ and having a large number of plants with complete rust resistance.Item Desempenho de híbridos F1 de café arábica com resistência simultânea a ferrugem, mancha aureolada e bicho mineiro(Editora UFLA, 2015-07) Andreazi, Elder; Sera, Gustavo Hiroshi; Faria, Ricardo Tadeu de; Sera, Tumoru; Shigueoka, Luciana Harumi; Carvalho, Filipe Gimenez; Carducci, Fernando César; Chamlet, DanielO objetivo deste estudo foi selecionar híbridos F1 com alta produtividade, elevado vigor vegetativo e com resistência simultânea à ferrugem, mancha aureolada e ao bicho-mineiro. Os experimentos foram instalados em três diferentes municípios do estado do Paraná: Londrina, na estação experimental do Instituto Agronômico do Paraná (IAPAR); Congonhinhas e Itaguajé. Foram avaliadas as características produtividade e vigor vegetativo, além da resistência à ferrugem, mancha aureolada e bicho mineiro, em 13 híbridos e em quatro cultivares comerciais do tipo linhagem, usadas como controle. Produtividade relativa, em relação à média da melhor testemunha foi estimada para todos os híbridos. Os híbridos 7, 11 e 12 apresentaram produtividade média significativamente maior do que as testemunhas em todos os locais. Os híbridos 6 e 9 foram simultaneamente resistentes à ferrugem, mancha aureolada e ao bicho- mineiro. O híbrido 12 foi resistente à ferrugem e à mancha aureolada. Os híbridos 6, 7, 9, 11 e 12 têm potencial para se tornarem cultivares de café.Item Resistência à ferrugem alaranjada em cultivares de café(Editora UFLA, 2010-01) Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Ito, Dhalton ShiguerAs cultivares de café Coffea arabica L. obtidas pelo Iapar eram resistentes à ferrugem Hemileia vastatrix quando foram registradas, mas com o surgimento de novas raças, essas cultivares apresentam, atualmente, diferentes níveis de resistência. Neste trabalho objetivou-se avaliar a resistência à ferrugem em 12 cultivares de café desenvolvidas pelo Iapar. A avaliação da resistência foi para a população local de raças de ferrugem em condições de alta intensidade da doença em campo e foi realizada em agosto de 2007 em Londrina - PR. Avaliaram-se 12 cultivares, desde IPR 97 a IPR 108. Como padrões de resistência e suscetibilidade, foram avaliados, respectivamente, ‘Iapar 59’ e ‘Catuaí Vermelho IAC 81’. O delineamento experimental foi em blocos ao acaso com nove repetições e parcelas de sete plantas. Para a avaliação da resistência, foi utilizada uma escala de notas que variava de 1 a 5, baseada na severidade da ferrugem. A avaliação da produtividade foi com base na colheita realizada em junho de 2007. As cultivares Iapar 59, IPR 97, IPR 98, IPR 104 e IPR 105 apresentaram resistência completa à ferrugem; ‘IPR 99’, ‘IPR 101’, ‘IPR 102’ e ‘IPR 107’ apresentaram cerca de 75% das plantas com resistência completa, assim, provavelmente, gene(s) de resistência estão em condição heterozigótica. As cultivares IPR 103 e IPR 108 podem ter resistência incompleta. A cultivar IPR 100 apresentou suscetibilidade similar ao da cultivar Catuaí Vermelho IAC 81; a ‘IPR 106’ também pode ser suscetível.