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URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352
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Item Assisted selection using molecular markers linked to rust resistance SH3 gene in Coffea arabica(Crop Breeding and Applied Biotechnology, 2023-10-25) Silva, Angelita Garbossi; Ariyoshi, Caroline; Shigueoka, Luciana Harumi; Pereira, Luiz Filipe Protasio; Sera, Gustavo HiroshiThe aim of this work was to validate markers linked to the SH3 gene of coffee leaf rust (CLR) resistance and use them in assisted selection. Initially, we validated the markers in genotypes already known to carry SH3. Next, we performed phenotype and genotype evaluation for resistance to CLR in coffee plants growing under field conditions. We used Arabica coffee progenies derived from BA-10, which carries the SH3 gene due to introgression of C. liberica. Three SCAR markers (SP-M16-SH3, BA-48-21O-f, and BA-124-12K-f) and one SSR marker (Sat244) linked to SH3 gene were used to amplify the coffee plants’ DNA. Our assessments of markers validation in resistant genotypes, SP-M16-SH3 and BA-124-12K-f, were efficient to identify the SH3 gene. These two markers were used to evaluate the progenies derived from BA-10 and were significantly linked to the phenotype evaluations. The SP-M16-SH3 marker was more efficient, with the advantage of being codominant.Item Rust resistance in arabic coffee cultivars in northern Paraná(Instituto de Tecnologia do Paraná - Tecpar, 2013-01) Grossi, Leandro Del; Sera, Tumoru; Sera, Gustavo Hiroshi; Fonseca, Inês Cristina de Batista; Ito, Dhalton Shiguer; Shigueoka, Luciana Harumi; Andreazi, ElderThe objective of the study was to evaluate the resistance to rust in coffee cultivars developed by research institutes of Brazil in Paraná state. Resistance to the local leaf rust races was assessed in high disease intensity field conditions at Londrina and Congonhinhas in 2009 and 2010. The cultivars were developed by the EPAMIG/UFV, IAPAR, IAC and MAPA/Procafé. The resistant standard ‘IAPAR 59' and the susceptible standards Catuaí Vermelho IAC 144' and 'Bourbon Amarelo' were used. A randomized block design with three replications and plots with 10 plants was used. A scale from 1 to 5 based on the rust intensity was used to evaluate the resistance. The Catiguá MG 1, Catiguá MG 2, IAPAR 59, IPR 98, IPR 104, Palma II, Paraíso H-419-10-6-2-5-1, Paraíso H-419- 10-6-2-10-1, Paraíso H-419-10-6-2-12-1, Pau Brasil MG 1 and Sacramento MG 1 cultivars presented complete resistance to rust at Londrina and Congonhinhas. The cultivars derived from the Catucaí germplasm were susceptible or showed different levels of partial resistance. Partial resistance to the rust was observed in several coffees derived from “Hibrido de Timor”. 'Acauã' and 'Obatã IAC 1669-20' presented complete resistance at Londrina, but at Congonhinhas, they were partially resistant, indicating that different rust races have occurred at these two locations.Item Selection of Arabic coffee progenies with rust resistance(Crop Breeding and Applied Biotechnology, 2014-06) Shigueoka, Luciana Harumi; Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Mariucci Junior, ValdirThe aim of this study was to select high-yielding coffee progenies with resistance to coffee leaf rust for the State of Paraná (Brazil). Field experiments in a randomized block design were carried out in Itaguajé and Congonhinhas. Yield, vegetative vigor and rust resistance of nine progenies of Arabic coffee and three check cultivars were evaluated. Many genotypes derived from “Sarchi- mor” and “Catucaí” were susceptible. Three coffee genotypes of Sarchimor germplasm and the F 6 generation of genotype “Catuaí x (Catuaí x BA-10 coffee)” were selected to advance generations and are promising to become new cultivars for being higher-yielding than ‘IAPAR 59’ and ‘Tupi IAC 1669-33’ and having a large number of plants with complete rust resistance.Item Desempenho de híbridos F1 de café arábica com resistência simultânea a ferrugem, mancha aureolada e bicho mineiro(Editora UFLA, 2015-07) Andreazi, Elder; Sera, Gustavo Hiroshi; Faria, Ricardo Tadeu de; Sera, Tumoru; Shigueoka, Luciana Harumi; Carvalho, Filipe Gimenez; Carducci, Fernando César; Chamlet, DanielO objetivo deste estudo foi selecionar híbridos F1 com alta produtividade, elevado vigor vegetativo e com resistência simultânea à ferrugem, mancha aureolada e ao bicho-mineiro. Os experimentos foram instalados em três diferentes municípios do estado do Paraná: Londrina, na estação experimental do Instituto Agronômico do Paraná (IAPAR); Congonhinhas e Itaguajé. Foram avaliadas as características produtividade e vigor vegetativo, além da resistência à ferrugem, mancha aureolada e bicho mineiro, em 13 híbridos e em quatro cultivares comerciais do tipo linhagem, usadas como controle. Produtividade relativa, em relação à média da melhor testemunha foi estimada para todos os híbridos. Os híbridos 7, 11 e 12 apresentaram produtividade média significativamente maior do que as testemunhas em todos os locais. Os híbridos 6 e 9 foram simultaneamente resistentes à ferrugem, mancha aureolada e ao bicho- mineiro. O híbrido 12 foi resistente à ferrugem e à mancha aureolada. Os híbridos 6, 7, 9, 11 e 12 têm potencial para se tornarem cultivares de café.