Periódicos
URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352
Navegar
13 resultados
Resultados da Pesquisa
Item Resistance to bacterial halo blight in Arabica coffee lines derivative from the genotype C1195-5-6-2 under natural infection conditions(Crop Breeding and Applied Biotechnology, 2018) Andreazi, Elder; Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Shigueoka, Luciana Harumi; Santos, Willian Gabriel dos; Pereira, Carlos Theodoro MottaThe aim of this study was to identify resistance to bacterial halo blight in Arabica coffee lines carrying Coffea racemosa genes. Eighteen Arabica coffee lines derivative from the genotype C1195-5-6-2, and the cultivars IAPAR 59 and IPR 99 were evaluated for resistance to bacterial halo blight in two trials carried out in field conditions, in Londrina, PR, Brazil. The cultivars Mundo Novo IAC 376-4 and Catuaí Vermelho IAC 81 were included as susceptible controls. Ten lines and the cultivar IAPAR 59 showed resistance to bacterial halo blight. The cultivar IPR 99 presented intermediate reaction, and the controls were very susceptible. This is the first study to show that lines derivative from the genotype C1195-5-6-2, which has C. racemosa genes, could be a source of resistance to bacterial halo blight in coffee breeding programs.Item Assisted selection using molecular markers linked to rust resistance SH3 gene in Coffea arabica(Crop Breeding and Applied Biotechnology, 2023-10-25) Silva, Angelita Garbossi; Ariyoshi, Caroline; Shigueoka, Luciana Harumi; Pereira, Luiz Filipe Protasio; Sera, Gustavo HiroshiThe aim of this work was to validate markers linked to the SH3 gene of coffee leaf rust (CLR) resistance and use them in assisted selection. Initially, we validated the markers in genotypes already known to carry SH3. Next, we performed phenotype and genotype evaluation for resistance to CLR in coffee plants growing under field conditions. We used Arabica coffee progenies derived from BA-10, which carries the SH3 gene due to introgression of C. liberica. Three SCAR markers (SP-M16-SH3, BA-48-21O-f, and BA-124-12K-f) and one SSR marker (Sat244) linked to SH3 gene were used to amplify the coffee plants’ DNA. Our assessments of markers validation in resistant genotypes, SP-M16-SH3 and BA-124-12K-f, were efficient to identify the SH3 gene. These two markers were used to evaluate the progenies derived from BA-10 and were significantly linked to the phenotype evaluations. The SP-M16-SH3 marker was more efficient, with the advantage of being codominant.Item IPR 106: new Arabica coffee cultivar, resistant to some Meloidogyne paranaensis and M. incognita nematode populations of Paraná(Crop Breeding and Applied Biotechnology, 2020) Sera, Gustavo Hiroshi; Machado, Andressa Cristina Zamboni; Ito, Dhalton Shiguer; Shigueoka, Luciana Harumi; Silva, Santino Aleandro da; Sera, TumoruCultivar IPR 106 resulted from a spontaneous hybridization between “Icatu IAC 925” and an unknow dwarf plant. It is a dwarf cultivar with high rusticity, late ripening cycle, large grains, excellent cup quality and resistance to some populations of the nematodes Meloidogyne paranaensis and M. incognita found in the state of Paraná.Item Rust resistance in arabic coffee cultivars in northern Paraná(Instituto de Tecnologia do Paraná - Tecpar, 2013-01) Grossi, Leandro Del; Sera, Tumoru; Sera, Gustavo Hiroshi; Fonseca, Inês Cristina de Batista; Ito, Dhalton Shiguer; Shigueoka, Luciana Harumi; Andreazi, ElderThe objective of the study was to evaluate the resistance to rust in coffee cultivars developed by research institutes of Brazil in Paraná state. Resistance to the local leaf rust races was assessed in high disease intensity field conditions at Londrina and Congonhinhas in 2009 and 2010. The cultivars were developed by the EPAMIG/UFV, IAPAR, IAC and MAPA/Procafé. The resistant standard ‘IAPAR 59' and the susceptible standards Catuaí Vermelho IAC 144' and 'Bourbon Amarelo' were used. A randomized block design with three replications and plots with 10 plants was used. A scale from 1 to 5 based on the rust intensity was used to evaluate the resistance. The Catiguá MG 1, Catiguá MG 2, IAPAR 59, IPR 98, IPR 104, Palma II, Paraíso H-419-10-6-2-5-1, Paraíso H-419- 10-6-2-10-1, Paraíso H-419-10-6-2-12-1, Pau Brasil MG 1 and Sacramento MG 1 cultivars presented complete resistance to rust at Londrina and Congonhinhas. The cultivars derived from the Catucaí germplasm were susceptible or showed different levels of partial resistance. Partial resistance to the rust was observed in several coffees derived from “Hibrido de Timor”. 'Acauã' and 'Obatã IAC 1669-20' presented complete resistance at Londrina, but at Congonhinhas, they were partially resistant, indicating that different rust races have occurred at these two locations.Item Resistance to bacterial halo blight in Arabica coffee lines derivative from the genotype C1195-5-6-2 under natural infection conditions(Crop Breeding and Applied Biotechnology, 2018-01) Andreazi, Elder; Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Carducci, Fernando Cesar; Shigueoka, Luciana Harumi; Santos, Willian Gabriel dosThe aim of this study was to identify resistance to bacterial halo blight in Arabica coffee lines carrying Coffea racemosa genes. Eighteen Arabica coffee lines derivative from the genotype C1195-5-6-2, and the cultivars IAPAR 59 and IPR 99 were evaluated for resistance to bacterial halo blight in two trials carried out in field conditions, in Londrina, PR, Brazil. The cultivars Mundo Novo IAC 376-4 and Catuaí Vermelho IAC 81 were included as susceptible controls. Ten lines and the cultivar IAPAR 59 showed resistance to bacterial halo blight. The cultivar IPR 99 presented intermediate reaction, and the controls were very susceptible. This is the first study to show that lines derivative from the genotype C1195-5-6-2, which has C. racemosa genes, could be a source of resistance to bacterial halo blight in coffee breeding programs.Item IPR 100 – Rustic dwarf Arabica coffee cultivar with resistance to nematodes Meloidogyne paranaensis and M. incognita(Crop Breeding and Applied Biotechnology, 2017-03) Sera, Tumoru; Sera, Gustavo Hiroshi; Fazuoli, Luiz Carlos; Machado, Andressa Cristina Zamboni; Ito, Dhalton Shiguer; Shigueoka, Luciana Harumi‘IPR 100’ was derived from a crossing between “Catuaí” and a hybrid(“Catuaí” x BA-10 coffee). ‘IPR 100’ is a medium size dwarf cultivar with high rusticity, with late ripening cycle, and with resistance to Meloidogyne paranaensis and to some M. incognita populations.Item Selection of Arabic coffee progenies with rust resistance(Crop Breeding and Applied Biotechnology, 2014-06) Shigueoka, Luciana Harumi; Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Mariucci Junior, ValdirThe aim of this study was to select high-yielding coffee progenies with resistance to coffee leaf rust for the State of Paraná (Brazil). Field experiments in a randomized block design were carried out in Itaguajé and Congonhinhas. Yield, vegetative vigor and rust resistance of nine progenies of Arabic coffee and three check cultivars were evaluated. Many genotypes derived from “Sarchi- mor” and “Catucaí” were susceptible. Three coffee genotypes of Sarchimor germplasm and the F 6 generation of genotype “Catuaí x (Catuaí x BA-10 coffee)” were selected to advance generations and are promising to become new cultivars for being higher-yielding than ‘IAPAR 59’ and ‘Tupi IAC 1669-33’ and having a large number of plants with complete rust resistance.Item Histopathological characterization of Coffea arabica cultivar IPR 106 resistance to Meloidogyne paranaensis(Escola Superior de Agricultura "Luiz de Queiroz", 2019-09) Shigueoka, Luciana Harumi; Dorigo, Orazília França; Arita, Lucas Yuuki; Fonseca, Inês Cristina de Batista; Silva, Santino Aleandro da; Sera, Gustavo Hiroshi; Machado, Andressa Cristina ZamboniMeloidogyne species are widely distributed in coffee growing areas in Brazil, where M. paranaensis is responsible to significant losses to the crop. One of the main management strategies is the use of resistant cultivars, such as Apoatã rootstock and cultivars IPR 100 and IPR 106. However, the parasitic relationship between coffee and M. paranaensis in culti- vars carrying resistance genes has been poorly understood. The objective of this paper was to characterize the resistance response of Coffea arabica cv. IPR 106 to M. paranaensis. For this, penetration and histopathological studies were carried out using root tissues parasitized by the nematode. ‘IPR 106’ and ‘Mundo Novo’ (susceptible cultivar) seedlings were inoculated with 4,000 eggs of M. paranaensis and, 15 days after inoculation (DAI), roots were stained with fuch- sine to verify the penetration rates of the nematode. Histopathological studies were conducted at 15, 30, 45 and 60 DAI and nematode reproduction was calculated at 120 DAI. ‘IPR 106’ did not avoid completely nematode penetration at 15 DAI, although the number of nematodes was 50 % lower than in Mundo Novo, disregarding the hypothesis of a pre-infectional mechanism of resistance that could prevent nematode penetration completely. However, giant cells and col- lapsed nematodes were observed in ‘IPR 106’ at 30 DAI, suggesting that the resistance mecha- nism to M. paranaensis in ‘IPR 106’ involves a post-infectional response and could be mediated by a hypersensitive reaction.Item Seleção para a resistência à ferrugem em progênies das cultivares de café IPR 99 e IPR 107(Instituto Agronômico (IAC), 2010-07) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Fonseca, Inês Cristina de Batista; Kanayama, Fabio Seidi; Grossi, Leandro Del; Shigueoka, Luciana HarumiAs cultivares de café IPR 99 ("Sarchimor") e IPR 107 ('IAPAR 59' x 'Mundo Novo IAC 376-4') eram no passado resistentes à ferrugem (Hemileia vastatrix). Os objetivos deste estudo foram: a) Identificar progênies dessas duas cultivares com resistência à ferrugem; b) Identificar progênies com resistência incompleta; c) Verificar a eficiência dos cruzamentos testes na seleção dessas cultivares. A avaliação da resistência em campo foi realizada em cafeeiros adultos expostos à população local de raças presentes no IAPAR. Foram avaliados 23 cruzamentos testes com progênies F3 de 'IPR 107' e 5 com progênies F4 de 'IPR 99'. Além disso, foram avaliadas 11 progênies F4 de 'IPR 107' e 5 progênies F5 de 'IPR 99', provenientes de autofecundação. Várias progênies das cultivares IPR 99 e 107 apresentaram alta freqüência de plantas com resistência completa e são portadoras de mais genes de resistência não quebrados pelas raças de ferrugem. Progênies das cultivares IPR 99 e IPR 107 com a resistência quebrada apresentaram resistência incompleta à população local de raças. Cruzamentos testes foram eficientes na seleção de progênies de café com mais genes de resistência não quebrados.Item Reação de progênies de café arábica derivadas do Icatu a Meloidogyne paranaensis(Instituto Agronômico (IAC), 2016-04) Shigueoka, Luciana Harumi; Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Andreazi, Elder; Carvalho, Filipe Gimenez; Carducci, Fernando Cesar; Ito, Dhalton ShiguerO objetivo do trabalho foi avaliar a reação ao nematoide Meloidogyne paranaensis em progênies de café arábica derivadas do Icatu. O experimento foi conduzido em telado, no Instituto Agronômico do Paraná (IAPAR), em Londrina, Paraná, Brasil. Mudas com três a quatro pares de folhas foram inoculadas com 5.000 ovos e juvenis de segundo estádio (J2) de Meloidogyne paranaensis. Foram avaliadas quatro progênies F 4 de HN 87609 derivadas de ‘Icatu H4782-7-925’. Como padrões de suscetibilidade e resistência, foram utilizados, respectivamente, Coffea arabica cv. Catuaí Vermelho IAC 81 e C. arabica cv. IPR 100. O experimento foi instalado em blocos casualizados com 14 repetições e uma planta por parcela. As avaliações foram efetuadas 120 dias após a inoculação. Foram avaliados o número de ovos e J2 por gramas de raiz (Nematoides·g-¹ ) e o fator de reprodução (FR). Para classificar os níveis de resistência das progênies, foi utilizado o índice de suscetibilidade do hospedeiro (ISH). De acordo com Nematoides·g-¹ , as progênies IAPAR 12232 e IAPAR 12231 não diferiram estatisticamente do padrão resistente ‘IPR 100’. Todas as progênies F 4 de HN 87609 foram altamente resistentes pelo ISH e apresentaram 100% das plantas classificadas como altamente resistentes ou resistentes. Portanto, a resistência a M. paranaensis está condição homozigótica.