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URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352
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Resultados da Pesquisa
Item Comportamiento de oviposición de Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) sobre su huésped natural(Sociedade Entomológica do Brasil, 2010-12) López-Guillén, Guillermo; Infante, Francisco; Castillo, AlfredoPhymastichus coffea LaSalle is an African endoparasitoid of adults of the coffee berry borer, Hypothenemus hampei (Ferrari) that was introduced into Mexico for biological control purposes. The objective of this study was to investigate the oviposition behavior of P. coffea on its host under laboratory conditions. The results indicate that P. coffea females exhibited 12 behavioral acts during and after oviposition, such as, walking, cleaning, flying, surface landing, resting, host landing, host antennation, touching the host with forelegs, host recognition, walking on the host, oviposition and cleaning after oviposition. The sequence of each behavior is described in an ethogram.Item Efficiency of Metarhizium anisopliae in the control of Meloidogyne incognita in banana and coffee crops(Editora UFLA, 2021) Oliveira, Claudio Marcelo Gonçalves de; Rosa, Juliana Magrinelli Osório; Pillat, Rhayane Resende; Almeida, José Eduardo Marcondes deBanana and coffee are crops damaged by the root-knot nematode (Meloidogyne incognita). Thus, the objective of this research was to study the efficiency of biological products in the control of M. incognita in both crops. Four experiments were carried out in a greenhouse, in a completely randomized design, inoculating banana and coffee plants with 2000 eggs and second-stage juveniles of M. incognita. In experiment 1, each plot was constituted of a banana seedling cv. Prata Anã. The treatments, applied around each seedling, contained the fungi Metarhizium anisopliae, from the collection kept in the Biological Control/CAPSA, IB, and Pochonia chlamydosporia (the commercial product Rizotec). In experiment 2, coffee seedlings cv. Arara were treated in a single dose with M. anisopliae and P. chlamydosporia. In experiments 3 and 4, coffee seedlings cv. Mundo Novo were treated alone or mixed with M. anisopliaeand Purpureocillium lilacinum. Based on the results obtained after 150 days of inoculation, it was concluded that M. anisopliae and P. chlamydosporia have usage potential for biological control of M. incognita in bananas and coffee. In the coffee crop, P. chlamydosporia showed 81% efficiency in reducing the population of M. incognita, whereas for M. anisopliae, the efficiency was up to 76.9%. However, there was no difference between treatments with biological control agents in relation to fresh root weight and dry weight of the aerial part of the coffee plants.Item Hymenoptera associated with conilon coffee crops in Espírito Santo state, Brazil, with emphasis on the families Diapriidae, Platygastridae and Scelionidae(Editora UFLA, 2020) Oliveira, Bruno Garcia de; Benavides-López, Jose Luis; Pádua, Diego Galvão deInformation about parasitoid wasp fauna can help determine the most appropriate methods for maintaining the ecological balance in agroecosystems, as these organisms are efficient natural enemies of various agricultural pests. The aim of this study was to survey the Hymenoptera families in conilon coffee plantations intercropped with other crops, focusing on the Diapriidae, Platygastridae and Scelionidae families, in order to determine wasps that could possibly act as biological controls. The study was carried out in eight conilon coffee farms located in three municipalities (Linhares, Marilândia and Sooretama) in Espírito Santo state, Brazil, with field samples collected in May (dry season) and December (rainy season) 2014. Were collected 1084 Hymenoptera specimens distributed in 10 superfamilies and 25 families. Fourteen genera of Scelionidae were collected, with Telenomus (34%), Idris (15%), Trissolcus (14%) and Ceratobaeus (10%) as the most abundant genera; seven genera of Diapriidae were collected with Doliopria (41%), Basalys (22%) and Trichopria (20%) as the most abundant; and two genera of Platygastridae were collected: Leptacis (52%) and Synopeas (14%). The highest abundance of wasp individuals was found in coffee intercropped with rubber trees. Intercropped coffee cultivation could potentially attract more natural enemies, as well as egg parasitoids that are promising biological controls.Item Association of Heterorhabditis amazonensis and Beauveria bassiana aiming the control of Hypothenemus hampei(Editora UFLA, 2020) Moreira, Igor Bitencourt Lima; Andaló, Vanessa; Assis, Gleice Aparecida de; Faria, Lucas Silva de; Carvalho, Fabio Janoni; Zampiroli, RenanAmong the main problems for coffee production, it is possible to mention the occurrence of coffee borer, Hypothenemus hampei. In order to seek alternatives to control the pest, the proposed objective was to evaluate the interaction between Beauveria bassiana UFU01 and Heterorhabditis amazonensis MC01 on the control of coffee borer. Therefore, four experiments were set. In the first, B. bassiana UFU01, H. amazonensis MC01 and both entomopathogens associated were applied in bored fruits displaced in vermiculite. In the second experiment, the methodology was similar to the previous one; however, the fruits were immersed in the suspensions. In the third experiment was evaluated the transport of B. bassiana UFU01 conidia by H. amazonensis, checking the mortality of Tenebrio molitor. In the fourth experiment, the fungus and the nematode were added to Petri dishes containing agar-water simultaneously to observe if the nematodes could carry B. bassiana UFU01 conidia. The obtained data were subjected to analysis of variance and the Tukey test (p <0.05), for comparison between means and regression analysis. It was found that, when entomopathogens were applied to vermiculite, treatments using B. bassiana UFU01 and H. amazonensis alone caused the highest mortality of H. hampei adults. For larvae, there was no difference among treatments. The same was observed when the fruits were dipped in the suspensions, with no difference among treatments. As for T. molitor mortality, it was observed that there was no difference among treatments. However, when applied in combination, there was a higher mortality caused by B. bassiana compared to H. amazonensis. In the last experiment, juveniles and conidia were found on the opposite side of the dish, and it can be inferred that the presence of conidia was due to transport by infective juveniles.Item In vivo compatibility between Beauveria bassiana (Bals.) Vuillemin and castor oil on Hypothenemus hampei (Ferrari)(Editora UFLA, 2020) Celestino, Flávio Neves; Pratissoli, Dirceu; Santos Junior, Hugo José Gonçalves dos; Machado, Lorena ContariniThe degree of in vivo compatibility between the entomopathogenic fungi Beauveria bassiana (Bals.) Vuillemin (Ascomycota: Hypocreales) and castor oil is important, as the interaction can be additive, synergistic or antagonistic. Thus, the objective of this study was to evaluate the in vivo compatibility between castor oil and B. bassiana on the coffee berry borer. The CCA-UFES/Bb-4 isolate of B. bassiana was used in concentrations of 0.0 (control), 1 x 104, 1 x 105, 1 x 106 and 1 x 107 conidia mL-1. The concentrations of castor oil were: 0.0 (control), 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0% v v-1. The total and confirmed mortality were evaluated, and data submitted to variance analysis. To verify the effect of B. bassiana and castor oil concentrations, the data were submitted to regression analysis at 5% probability. Castor oil had an antagonistic effect on B. bassiana reducing the mortality of coffee berry borer. The mortality of H. hampei reduced caused by interaction between B. bassiana and castor oil, due to increasing concentration of castor oil. Lower interference of castor oil was observed for high concentrations of B. bassiana. Based on the results, management of H. hampei cannot be normally performed using the association between castor bean oil and B. bassiana, except if the economic viability of the higher concentrations justified.Item Effect of Bacillus spp. on Aspergillus westerdijkiae growth, sporulation and ochratoxin A production in green-coffee medium(Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2017-12) Einloft, Tiago Centeno; Oliveira, Patrícia Bolzan de; Veras, Flávio Fonseca; Welke, Juliane Elisa; Mallmann, Carlos Augusto; Dilkin, Paulo; Dionello, Rafael GomesAspergillus westerdijkiae is one of the most important spoilage and toxigenic fungi contaminating coffee beans and may produce ochratoxin A (OTA), a mycotoxin that characterize a health risk to the coffee consumers. Biological control strategies can be used for prevention of fungal invasion and decrease mycotoxin exposure. The aims of this study were to evaluate the in vitro effect of three Bacillus sp. biocontrol candidates on A. westerdijkiae mycelial growth, spore counts and OTA production. A green-coffee based medium was inoculated with A. westerdijkiae and Bacillus spp. (B. safensis RF69, B. amyloliquefaciens RP103 and B. subtilis RP242) and after incubation, the fungal growth, sporulation and mycotoxin production was evaluated. Mycelial growth rate was reduced in a range between 76-95% and conidial production was also significantly decreased. All isolates were capable of reducing OTA production in a range between 62-96%. The results showed that the biocontrol candidates may be an effective control method for A. westerdijkiae and OTA in coffee.