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URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352

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2004 - 20102

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Assunto: search.filters.subject.Molecular marker

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Agora exibindo 1 - 2 de 2
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    Development of microsatellite markers for identifying Brazilian Coffea arabica varieties
    (Sociedade Brasileira de Genética, 2010) Vieira, Elisa S.N.; Pinho, Édila V. de R. Von; Carvalho, Maria G.G.; Esselink, Danny G.; Vosman, Ben
    Microsatellite markers, also known as SSRs (Simple Sequence Repeats), have proved to be excellent tools for identifying variety and determining genetic relationships. A set of 127 SSR markers was used to analyze genetic similarity in twenty five Coffea arabica varieties. These were composed of nineteen commercially important Brazilians and six interspecific hybrids of Coffea arabica, Coffea canephora and Coffea liberica. The set used comprised 52 newly developed SSR markers derived from microsatellite enriched libraries, 56 designed on the basis of coffee SSR sequences available from public databases, 6 already published, and 13 universal chloroplast microsatellite markers. Only 22 were polymorphic, these detecting 2-7 alleles per marker, an average of 2.5. Based on the banding patterns generated by polymorphic SSR loci, the set of twenty-five coffee varieties were clustered into two main groups, one composed of only Brazilian varieties, and the other of interspecific hybrids, with a few Brazilians. Color mutants could not be separated. Clustering was in accordance with material genealogy thereby revealing high similarity.
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    Varietal identification of coffee seeds by RAPD technique
    (Instituto de Tecnologia do Paraná - Tecpar, 2004-03) Crochemore, Maria Lúcia; Nunes, Liliane Moreira; Andrade, Giselly Aparecida; Molinari, Hugo Bruno Correa; Vasconcellos, Maria Elizabeth
    This study aimed the identification of cultivars and/or lines of Coffea arabica of commercial interest, using PCR-RAPD markers. The DNA of ground seeds lots of 12 cultivars and/or lines were evaluated with five primers (Operon OPA 01, OPA 04, OPG 11, OPY 16, and OPX 09) were obtained from a selection of 56 primers. The electrophoretic profiles allowed distinction among eight cultivars and/or lines as well as heterogeneity between and within lots of IAPAR59.