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Item Resistance to bacterial halo blight in Arabica coffee lines derivative from the genotype C1195-5-6-2 under natural infection conditions(Crop Breeding and Applied Biotechnology, 2018) Andreazi, Elder; Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Shigueoka, Luciana Harumi; Santos, Willian Gabriel dos; Pereira, Carlos Theodoro MottaThe aim of this study was to identify resistance to bacterial halo blight in Arabica coffee lines carrying Coffea racemosa genes. Eighteen Arabica coffee lines derivative from the genotype C1195-5-6-2, and the cultivars IAPAR 59 and IPR 99 were evaluated for resistance to bacterial halo blight in two trials carried out in field conditions, in Londrina, PR, Brazil. The cultivars Mundo Novo IAC 376-4 and Catuaí Vermelho IAC 81 were included as susceptible controls. Ten lines and the cultivar IAPAR 59 showed resistance to bacterial halo blight. The cultivar IPR 99 presented intermediate reaction, and the controls were very susceptible. This is the first study to show that lines derivative from the genotype C1195-5-6-2, which has C. racemosa genes, could be a source of resistance to bacterial halo blight in coffee breeding programs.Item MANAGEMENT OF BACTERIAL HALO BLIGHT AND OTHER COFFEE CROP DISEASES(Sociedade Brasileira de Fitopatologia (SBF), 2021) Patricio, Flávia Rodrigues Alves; Beriam, Luis Otávio Saggion; Santos, José Maria Fernandes dos; Moraes, Arivaldo de; Rossi, Agnaldo; Reis, Joao Carlos Seixas; Almeida, Irene Maria Gatti deBacterial halo blight (BHB), caused by Pseudomonas syringae pv. garcae, has reemerged as an important disease in Brazil, especially in coffee cultivated at high altitude in the states of Minas Gerais and São Paulo. In this study we evaluated copper-based antimicrobial compounds (CBACs), the antibiotic kasugamycin and the resistant inducers acibenzolar-S-methyl (ASM) and phosphite for BHB management in four experiments carried out in coffee crops in the municipalities of Caconde and Altinópolis, São Paulo State, Brazil. Fungicides to control brown leaf spot (BLS), a disease caused by Boeremia exigua pv. coffea, were also included in two experiments, because both diseases frequently occur simultaneously. Copper oxychloride, copper hydroxide, antibiotic, and ASM mixture with copper hydroxide, and phosphite reduced BHB incidence and had no phytotoxic effects on flowers or pin-head berries. Mixtures of boscalid or pyraclostrobin with copper hydroxide were compatible and effective for the simultaneous control of BHB and BLS. In this study, we showed that August-September is the most important period to control BHB in Brazil and lasts until December, when disease incidence increases, and flowers and pin-head berries are being formed. The chemical management of BHB in this period can improve the coffee crop yield.Item Multiple resistance to bacterial halo blight and bacterial leaf spot in Coffea spp.(Instituto Biológico, 2019) Rodrigues, Lucas Mateus Rivero; Destéfano, Suzete Aparecida Lanza; Almeida, Irene Maria Gatti de; Beriam, Luís Otávio Saggion; Braghini, Masako Toma; Guerreiro Filho, OliveiroBreeding for genetic resistance is an important method of crop disease management, due to the numerous benefits and low cost of establishment. In this study, progenies of 11 Coffea species and 16 wild C. arabica accessions were tested for their response to Pseudomonas syringae pv. garcae, the causal agent of bacterial halo blight, a widespread disease in the main coffee-producing regions of Brazil and considered a limiting factor for cultivation in pathogen-favorable areas; and also to P. syringae pv. tabaci, causal agent of bacterial leaf spot, a highly aggressive disease recently detected in Brazil. Separate experiments for each disease were carried out in a greenhouse, with artificial pathogen inoculations and ideal moisture conditions for disease development. The results showed that C. canephora, C. congensis, C. eugenioides, C. stenophylla, and C. salvatrix progenies, the wild C. arabica accessions Dilla & Alghe and Palido Viridis, and cultivar IPR 102 contain satisfactory levels of simultaneous resistance against bacterial halo blight and bacterial leaf spot. These results are useful in breeding programs for durable resistance to multiple biotic agents, providing new combinations of resistance alleles by hybridization, as well as for phytopathological studies, to identify infraspecific variability of the pathogens.Item Can the leaf age influence the susceptibility to bacterialleaf- spot and bacterial-halo-blight on coffee seedlings?(Instituto Biológico, 2018) Tomiyama, Ana Laura Midori Rossi; Rodrigues, Lucas Mateus Rivero; Andrade, Vinicius Teixeira; Beriam, Luis Otavio Saggion; Destéfano, Suzete Aparecida Lanza; Guerreiro Filho, OliveiroPseudomonas syringae van Hall, 1902, causes yield losses in innumerous economic important crops. On coffee trees, P. syringae pv. garcae causes the bacterial-halo-blight (BHB) and P. syringae pv. tabaci the bacterial-leaf-spot (BLS). Recently, these diseases incidence has increase in occurrence areas and aggressiveness in Brazil. Although leaf age plays a role in the severity response of BHB, it is not known yet if this phenomenon also occurs in coffee-BLS interaction, and with highly virulent strains. So, we examined differences in the diseases severity by inoculation of P. syringae pv. garcae and P. syringae pv. Tabaci strains on coffee leaves with different ages, to compare this aspect with coffee-BLS interaction. Our results showed that, for both pathovars, the severity was greater at the first internodes leaves, although for the most aggressive strains it was quite similar on any leaf age.Item Diferenciação de bactérias do gênero Pseudomonas patogênicas ao cafeeiro por técnicas serológicas(Instituto Biológico, 2017) Beriam, Luis Otavio Saggion; Patrício, Flavia Rodrigues Alves; Maciel, Karen Wolf; Rodrigues, Lucas Mateus Rivero; Almeida, Irene Maria Gatti deHá várias bactérias que causam problemas para o cafeeiro, incluindo Pseudomonas cichorii, P. syringae pv. garcae, P. syringae pv. tabaci, Burkholderia andropogonis e Xylella fastidiosa, todas elas já descritas no Brasil. Tentativas de diferenciar essas bactérias por testes serológicos de dupla difusão em ágar (dda), com antissoros produzidos contra células íntegras de P. s. pv. garcae, mostraram reações cruzadas, principalmente entre P. s. pv. garcae e P. s. pv. tabaci. Dessa forma, foram produzidos antissoros contra P. s. pv. garcae (linhagem patotipo IBSBF-248 — Coleção de Culturas de Fitobactérias do Instituto Biológico — IBSBF), obtidos por meio de imunizações de coelhos com antígenos de proteínas do complexo proteico da membrana (CPM). Esses antissoros foram testados por dupla difu são em agarose (dda) contra diversas formas de antígenos extraídos de P. cichorii, P. s. pv. garcae e P. s. pv. tabaci [células autoclavadas, células tratadas com formol, exopolissacarídeos (EPS), glicoproteí nas (GP) da cápsula bacteriana, proteínas de membrana e suspen são bacteriana (SB) em NaCl 0,85%]. Os resultados mostraram que, dependendo do antígeno e do meio suporte da dupla difusão (com ou sem MgCl2 e/ou azul de tripano), os antissoros reagem somente com P. s. pv. garcae. Desse modo, esses antígenos podem ser usados para a rápida diagnose da mancha aureolada do cafeeiro nos testes de dda.Item Geostatistical analysis of bacterial blight in coffee tree seedlings in the nursery(Grupo Paulista de Fitopatologia, 2018) Belan, Leônidas Leoni; Pozza, Edson Ampélio; Alves, Marcelo de Carvalho; Freitas, Marcelo Loran de OliveiraIn nurseries of coffee tree seedlings (Coffea arabica), there are favorable conditions for bacterial blight epidemics (Pseudomonas syringae pv. garcae). Studying the spatial distribution of diseased plants can help in the adoption of management strategies. Likewise, geostatistics has been applied to shape the spatial distribution and study epidemiological aspects of plant diseases. Thus, this study was developed to characterize the spatial distribution pattern of bacterial blight in a nursery of coffee tree seedlings. The disease progress was monitored over time in 704 seedlings organized in lines and columns in a nursery. Considering the mean diameter of the pots used for producing seedlings, georeferencing was carried out in Cartesian coordinate system for the seedlings in the nursery. The disease incidence data were subjected to non-spatial exploratory analysis and geostatistical analysis. The spherical isotropic semivariogram model was adjusted to the data and data interpolation was performed by ordinary kriging to visualize the spatial distribution of symptomatic seedlings. Bacterial blight epidemic was detected in the nursery during the experimental period, and there was variability and spatial dependence in relation to the distribution of diseased seedlings. As the epidemic progressed, the population of diseased plants increased, as well as the number and the size of the foci and their coalescence. Besides, there was an increase in the range value, sill and nugget effect. The kriging maps showed the disease progress and its variance. The bacterial blight epidemic of coffee tree started with a random spatial distribution pattern, progressing to an aggregate pattern.Item Resistance to bacterial halo blight in Arabica coffee lines derivative from the genotype C1195-5-6-2 under natural infection conditions(Crop Breeding and Applied Biotechnology, 2018-01) Andreazi, Elder; Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Carducci, Fernando Cesar; Shigueoka, Luciana Harumi; Santos, Willian Gabriel dosThe aim of this study was to identify resistance to bacterial halo blight in Arabica coffee lines carrying Coffea racemosa genes. Eighteen Arabica coffee lines derivative from the genotype C1195-5-6-2, and the cultivars IAPAR 59 and IPR 99 were evaluated for resistance to bacterial halo blight in two trials carried out in field conditions, in Londrina, PR, Brazil. The cultivars Mundo Novo IAC 376-4 and Catuaí Vermelho IAC 81 were included as susceptible controls. Ten lines and the cultivar IAPAR 59 showed resistance to bacterial halo blight. The cultivar IPR 99 presented intermediate reaction, and the controls were very susceptible. This is the first study to show that lines derivative from the genotype C1195-5-6-2, which has C. racemosa genes, could be a source of resistance to bacterial halo blight in coffee breeding programs.Item Kasugamycin influence on bacterial blight of coffee and on green coffee beans physicochemical quality(Editora UFLA, 2018-01) Barbosa, Ronei Aparecido; Santini, Paula Tristão; Guilherme, Luiz Roberto GuimarãesBrazil stands out as the world’s largest coffee exporter. However, in the cold and windy regions, such as the southern of Minas Gerais, have been undergoing attack of diseases, including the bacterial blight of coffee caused by the bacterium Pseudomonas syringae pv. garcae, which despite not having the same importance as the coffee leaf rust, is causing damages and losses to the coffee plantation. The control is mainly done through preventive measures such as installation of windbreaks. When bacterial blight of coffee is already installed, the chemical control is used with syrups of copper-based products and antibiotics. The aim of the present study was to verify the efficiency of the antibiotic kasugamycin associated with copper hydroxide in the control of bacterial blight of coffee and raw coffee beans quality. The experimental design was a randomized block with five treatments, four replicates and plots of 10 plants in the field where the treatments consisted of different concentrations of the kasugamycin: 0.0, 250, 500, 750 and 1000 mL ha-1 added with 1.0 L copper hydroxide. The percent analysis was performed on the raw beans in order to verify their physical and chemical quality. The obtained results demonstrate that the solution is efficient in the control of bacterial blight of coffee as well as in the improvement of vegetative vigor and production, but does not influence the quality of raw coffee bean.Item SH1 leaf rust and bacterial halo blight coffee resistances are genetically independent(Instituto Agronômico (IAC), 2017-04) Rodrigues, Lucas Mateus Rivero; Braghini, Masako Toma; Guerreiro Filho, OliveiroCoffee resistance to Pseudomonas syringae pv. garcae has been associated to pleiotropic effect of SH1 allele, present in coffee plants resistant to certain races of Hemileia vastatrix, the causal agent of leaf rust, or genetic linkage between resistance alleles to both pathogens. To validate this hypothesis, 63 coffee plants in F2 generation were evaluated for resistance to 2 isolates of H. vastatrix carriers of alleles, respectively, v2, v5 (isolate I/2015) and 1; v2; v5 (isolate II/2015) with the objective to confirm presence of SH1 allele in resistant plants to isolate I/2015. The same coffee plants were evaluated for resistance to a mixture of P. syringae pv. garcae strains highly pathogenic to coffee. Results showed that, among F2 coffee allele SH1 carriers, resistant to isolate I/2015, resistant and susceptible plants to bacterial halo blight were found; the same segregation occurs between F2 homozygous for SH1 allele, susceptible to the same isolate (I/2015) of H. vastatrix. Results also indicate that there is no pleiotropic effect of gene or allele SH1 connection between genes conferring resistance to leaf rust caused by H. vastatrix and bacterial halo blight caused by P. syringae pv. garcae.Item Bacterial halo blight of coffee crop: aggressiveness and genetic diversity of strains(Instituto Agronômico (IAC), 2018-01) Maciel, Karen Wolf; Destefano, Suzete Aparecida Lanza; Beriam, Luis Otavio Saggion; Almeida, Irene Maria Gatti de; Patricio, Flavia Rodrigues Alves; Rodrigues, Lucas Mateus Rivero; Guerreiro Filho, OliveiroBacterial halo blight, caused by Pseudomonas syringae pv. garcae, is an important disease of coffee crop occurring in Brazil and other countries. In recent years, outbreaks of this disease have damaged several coffee crops in Brazil. Aggressiveness and genetic diversity of 25 strains of P. s. pv. garcae, obtained between the years 1958 and 2011, in 23 cities of São Paulo and Minas Gerais states, as well as three strains from Kenya were evaluated in this study. The strains were inoculated on coffee seedlings cultivar Mundo Novo, and their genetic diversity was evaluated by ERIC-PCR, REP-PCR, and their combination. All the strains were pathogenic to the coffee seedlings; the results of pathogenicity tests, in both experiments, could be divided in four aggressiviness classes (highly aggressive; aggressive; moderately aggressive and less aggressive). The Kenyan strains grouped separately from the Brazilian strains with ERIC-PCR and the combination of ERIC- and REP-PCR. The Brazilian strains could be grouped in two sub-clusters, the first including the older strains, obtained from 1958 to 1978, and the other comprising the remaining strains. With a few exceptions, strains isolated from 1997 to 2011, grouped mainly by their region of origin, were predominantly isolated from higher altitude regions, above 800 m. This probably occurred because the climatic conditions that prevail in these regions, characterized by milder temperatures and regular rainfall, are favorable for the coffee crop and for the production of high quality coffee beverage, but can be also favorable to bacterial halo blight.