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URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3352
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Item Production of Coffea canephora seedlings through cuttings in a nursery and hydroponics using different containers(Universidade Federal de Lavras, 2023-07-19) Lima, Amador Eduardo de; Castro, Elisa de Melo; Cunha, Samuel Henrique Braga da; Guimarães, Rubens José; Chalfun, Nilton Nagib Jorge; Carvalho, Alex Mendonça de; Alves, Eduardo; Carvalho, Milene Alves FigueiredoThe production of seedlings is a determining factor for the establishment and success of the coffee plantation. The objective of this study was to evaluate the production of Coffea canephora seedlings by cuttings in a modified hydroponic system and in a conventional nursery, using different containers. At the end of the experiment, growth (height, stem diameter, total number of pairs of leaves, leaf area, root area, dry matter weight of leaves, stem and root), physiological (chlorophyll a, b and total and stomatal conductance) and anatomical (stomatal density, functionality and opening) characteristics were analyzed. For statistical analysis, a completely randomized design (CRD) was used, with five treatments and six replications, with ten plants per plot. Seedlings produced in a modified hydroponic system using 50- and 120-cm³ tubettes show better vegetative growth and physiological characteristics compared to those produced in a nursery. Seedling management in a modified hydroponic system using 120-cm³ tubettes provides greater cutting survival percentage, number of remaining leaves and emission of shoots. In nurseries, the use of polyethylene bags provides better seedling quality and survival in relation to the use of tubettes, regardless of volume.Item Regeneration and development of Coffea arabica L. plants through indirect somatic embryogenesis(Universidade Federal de Lavras, 2023-03-10) Arimarsetiowati, Rina; Daryono, Budi Setiadi; Astuti, Yohana Theresia Maria; Prastowo, Erwin; Semiarti, EndangMicropropagation of AS2K clones Arabica coffee (Coffea arabica L.) was attempted through indirect somatic embryogenesis by using ten different parts of the leaf such as shoot, first leaf base, second leaf base, third leaf base, first leaf middle, second leaf middle, third leaf middle, first leaf tip, second leaf tip, and third leaf tip. The influence of the part of leaf explants, combination of plant growth regulator (PGRs) in the induction of embryogenic callus and regeneration of embryo somatic were studied. Furthermore, the various protocols to induce regeneration of somatic embryo into plantlet through different step of subculture and the use of various germination medium were demonstrated. The morphological characteristics and histological analysis of embryogenic callus and embryo development were observed. In this experiment, it was observed that the M5 medium supplemented with 1 mg/L 2,4-D, 1 mg/L BAP and 4 mg/L 2-ip was closely associated with third leaf tip explants for induction of embryogenic callus. The maximum number of globular, heart-shape, torpedo and cotyledones (18, 4, 12, 4, respectively) were achieved on ERM6 medium containing 2 mg/L BAP without activated charcoal on 90th day for regeneration of embryo somatic. The length of roots is the most influence paramater on plantlet regeneration, and the 17th protocol which used B medium, large embryos and twice phase of subculture from liquid medium to solid medium is the best protocol for plantlet regeneration. The protocol developed could be useful highly for large-scale micropropagation in these commercially important Arabica coffee clones