Coffee Science
URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3355
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Item Effect of tropical millipede Rhinocricus botocudus in the degradation and maturity of coffee residues(Editora UFLA, 2021) Silva, Victor Maurício da; Passos, Renato Ribeiro; Marin, Ericka Broetto; Mendonça, Eduardo de SáIn Brazil, approximately 3 tons of dry mass per hectare of coffee residues (CR), formed by leaves and branches <5 mm in diameter, were accumulated around the crops in the last harvests of the semi-mechanized crops. Thus, the production of substrates and organic fertilizers from the bioconversion of residues of coffee activity is an important theme in Brazil and in the world. The objective of the study was to investigate the effectiveness of the tropical millipede Rhinocricus botocudus on the degradation and maturity of coffee residues (CR). The presence or absence of R. botocudus were tested in five sampling times (0, 30, 60, 90, and 120 days) with three replicates (n = 3). The analyzed variables were: total C and N contents, lignin, cellulose, soluble C and N, soluble polyphenols, C of humic substances, and infrared analysis of humic acid (HA) and fulvic acid (FA). At the final incubation stage (120 days), the lowest values of cellulose, cellulose/lignin, and polyphenols occurred with R. botocudus. Infrared analysis of HA demonstrated that readily biode gradable structures, such as alcohols and short chain aliphatic molecules, were decomposed over time, consequently reflecting the increase of aromatic structures of greater stability. Compared to the control, these changes in HA were more evident in the presence of R. botocudus. The results of the present study suggest that this millipede species modify the dynamics of degradation and humification of CR by accelerating the maturity of this residue.Item Isolation and identification of a native microbial consortium for the coffee pulp degradation above 2000 masl(Editora UFLA, 2021) Cuba, Marcela N. Arteaga; Dilas-Jiménez, Josué O.; Díaz, Norberto Villanueva; Miranda, Ofelia C. Cernaqué; Santillán, Segundo M. Tafur; García, José Enrique Olivera; Mugruza-Vassallo, Carlos A.Pulp and mucilage constitute the most abundant by-products of the processing of coffee and inadequate handling produces high-rates of pollution. Considering that in nature there is a large variety of cellulolytic microorganisms which can intervene in the degradation of coffee pulp, the inclusion of a native microbial cellulolytic consortium was assessed. The sample for isolation consisted of coffee pulp and soil from a humid forest at more than 2,000 masl. Nutrient agar was used as a means of isolation for bacteria, and malt agar for fungi, supplemented with 50% soil extract and 1% carboxylmethylbcellulose. This achieved the isolation of 118 strains of bacteria and 114 of fungi. Cellulolytic activity was established using the filter paper test, assessing and selecting only those that presented higher glucose production, among them 12 strains of fungi and 11 strains of bacteria. To obtain the microbial consortium, randomized blends were performed for both fungi and bacteria, again assessing the production of glucose. The bacterial consortium was made up of Ochrobactrum pseudo grignonense, Paenibacillus lauruscon and Bacillus xiamenensis and the fungal consortium by Fusarium sp., Penicillum sp., Cylindrocarpon sp. The optimal treatment achieved a complete degradation of the pulp in 28 days, that would contribute to the recovery and conservation of the coffee ecosystem. The main interpretation was that a wet environment at more than 2,000 masl is still suitable for coffee composting but different bacterial and fungi consortia were found to support other recent work done with one type of consortium.