Coffee Science
URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3355
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Resultados da Pesquisa
Item Lactic acid bacteria diversity and dynamics in Colombian coffee fermentation(Universidade Federal de Lavras, 2023-10-03) O'Byrne, Rosmery Cruz; Piraneque-Gambasica, Nelson; Aguirre-Forero, SoniaLactic acid bacteria (LAB) are recognized in coffee fermentation as key microorganisms in forming flavor and aroma precursors associated with high-quality beverages. In Colombia, although coffees with differential sensory characteristics are produced from one region to another, only some studies have been performed on the microbiology of coffee fermentation, of which no research focused on LAB species has been reported. In this study, LAB diversity and dynamics associated with coffee fermentation in the Sierra Nevada de Santa Marta (SNSM) were determined through a temporal high-throughput sequencing approach, where the 16S rRNA gene was amplified and sequenced using the Illumina MiSeq platform. Finally, LAB species were identified using the BLASTN algorithms of the NCBI GenBank. The coffee fermentation process that lasted 36 hours was dominated by the genera Leuconostoc, followed by Lactobacillus and Weissella. Of the 118 OTUs corresponding to LAB, it was possible to identify 50 bacterial species, among which 28 are reported for the first time in coffee fermentation. Among the species widely reported in coffee fermentation are Leuconostoc mesenteroides, Leuconostoc pseudomesenteroides, Lactiplantibacillus plantarum (basonym: Lactobacillus plantarum), Levilactobacillus brevis (basonym: Lactobacillus brevis), and Lactococcus lactis. While the novel reports mainly correspond to species belonging to genera that were previously recognized as Lactobacillus, such as Lactiplantibacillus, Paucilactobacillus, Secundilactobacillus, Liquorilactobacillus, Lacticaseibacillus, Schleiferilactobacillus, Loigolactobacillus, Ligilactobacillus, Lentilactobacillus, Limosilactobacillus, and Latilactobacillus. These findings suggest that the indigenous LAB of the SNSM are responsible for generating metabolites that develop specific characteristics of coffee in the region, which is why coffee from the SNSM is protected by designation of origin. Isolates of the reported species should be considered for application as starter cultures.Item Regeneration and development of Coffea arabica L. plants through indirect somatic embryogenesis(Universidade Federal de Lavras, 2023-03-10) Arimarsetiowati, Rina; Daryono, Budi Setiadi; Astuti, Yohana Theresia Maria; Prastowo, Erwin; Semiarti, EndangMicropropagation of AS2K clones Arabica coffee (Coffea arabica L.) was attempted through indirect somatic embryogenesis by using ten different parts of the leaf such as shoot, first leaf base, second leaf base, third leaf base, first leaf middle, second leaf middle, third leaf middle, first leaf tip, second leaf tip, and third leaf tip. The influence of the part of leaf explants, combination of plant growth regulator (PGRs) in the induction of embryogenic callus and regeneration of embryo somatic were studied. Furthermore, the various protocols to induce regeneration of somatic embryo into plantlet through different step of subculture and the use of various germination medium were demonstrated. The morphological characteristics and histological analysis of embryogenic callus and embryo development were observed. In this experiment, it was observed that the M5 medium supplemented with 1 mg/L 2,4-D, 1 mg/L BAP and 4 mg/L 2-ip was closely associated with third leaf tip explants for induction of embryogenic callus. The maximum number of globular, heart-shape, torpedo and cotyledones (18, 4, 12, 4, respectively) were achieved on ERM6 medium containing 2 mg/L BAP without activated charcoal on 90th day for regeneration of embryo somatic. The length of roots is the most influence paramater on plantlet regeneration, and the 17th protocol which used B medium, large embryos and twice phase of subculture from liquid medium to solid medium is the best protocol for plantlet regeneration. The protocol developed could be useful highly for large-scale micropropagation in these commercially important Arabica coffee clonesItem Genetic variability of conilon coffee population from cultivar ‘ES8152’ based on morphoagronomic variables(Universidade Federal de Lavras, 2022-06-09) Senra, João Felipe de Brites; Silva, Josimar Aleixo da; Ferrão, Maria Amélia Gava; Esposti, Marlon Dutra Degli; Milheiros, Idalina Sturião; Fassarella, Kamila MachadoThis study aimed to analyze the genetic variability of Coffea canephora population with 190 genotypes from cultivar ‘ES8152’, based on morphoagronomic characteristics and vegetation index, to identify the most important characteristics for genetic divergence and compare them with commercial clones. The experiment was installed, in 2019, at the Bananal do Norte Experimental Farm/INCAPER, Cachoeiro de Itapemirim, ES, Brazil. The experiment was carried out in Federer’s augmented block design with three blocks, four common treatments (commercial clones A1, LB1, V8 and V12) and 190 regular treatments, genotypes from the seed production field of the conilon coffee cultivar ‘ES8152’. At 24 months of age 14 morphoagronomic characteristics and vegetation index were evaluated. Descriptive analysis of the data, the estimation of the Standardized Euclidean Distance (ED) followed by the group-ing by the methods of Tocher, UPGMA and principal coordinates, in addition to the relative importance of the characters estimated by the Singh meth-odology were performed. The most distant genotypes were 62 and 83 (ED=2.620) and the closest were 42 and 160 (ED=0.208). Genotype 83 stood out as the most distant among the others. The optimization and hierarchical groupings allowed the identification of genotypes 15, 81, 107 and 184 as similar to commercial clones. The discard analysis of variables recommended the elimination of the vegetation index and average internode length of the next diversity analysis. Principal coordinate analysis found phenotypic similarity of the genotypes 30, 81, 115, 141 and 163 with the clone V12, of the genotype 119 with the clone A1 and genotype 17 with clone LB1. The study, of morphoagronomic characters, allowed to detection the genetic diversity existing in the materials evaluated, indicating those with phenotypic similarity with the commercial clones, being possible the early identification of promising genotypes, agronomically superior, to start a breeding program for clonal selection, recurrent selection and controlled crosses to maximize heterosis.Item Factor analysis for plant and production variables in Coffea canephorain the Western Amazon(Universidade Federal de Lavras, 2022-06-09) Silva, Gabi Nunes; Barroso, Laís Mayara Azevedo; Cruz, Cosme Damião; Rocha, Rodrigo Barros; Ferreira, Fábio MedeirosThe evaluation of morphological characters related to the hulled coffee yield subsidizes the selection of Coffea canephora plants that combine a set of favorable traits. However, the greater the number of traits considered, the more difficult the selection process becomes. In this context, multivariate analyzes can be useful to overcome this problem. The aim of this study was to identify, in a set of agronomic traits of Coffea canephora, the determining factors of biological phenomena and use these factors to recognize patterns of diversity and similarity from biological complexes of interest to the breeder. To this, eleven morphological descriptors were evaluated of 130 clones of the botanical varieties Conilon and Robusta and intervarietal hybrids over two crop years in the experimental field of Embrapa, in the municipality of Ouro Preto do Oeste, state of Rondônia (RO). To group the traits, the multivariate technique of Factor Analysis was used. The effect of genotype x year interaction was significant for the eleven traits analyzed. Based on the scree plot, three factors were established. Factors were interpreted as architecture, vigor and grains with a satisfactory percentage of explained variability. The inter-pretation of the factors highlighted the importance of the Conilon variety to improve the architecture of the Robusta botanical variety. These results show that it is possible to use factor scores to identify varieties and traits that favor higher production of hulled coffee.Item Evaluation of agronomic performance of coffee (Coffea arabica L.) cultivars in Gamo and Gofa, southern Ethiopia(Editora UFLA, 2021) Zebire, Degife; Fetena, Seifu; Shara, SaburaEthiopia is identified as a primary centre of Arabica coffee plant origin and genetic diversity. Climatic variations, varietal differences, soil type, mulching, shade, the farming method used, pruning, and other cultural practices are the major factors that affect the development of plants including coffee. Amongst the various factors for having a good yield of coffee is growing of coffee plant in its specific agro-ecologies which has a great contribution to both the life span of the coffee trees and its yield. This experiment was conducted to evaluate the performance of different released and local Arabica coffee varieties across two locations at Gamo and Gofa zones, Southern Ethiopia. The varieties which were used for the study were transplanted in 2014 and laid out in randomized complete block design with three replications across locations. The varieties were planted at a spacing of 2m by 2m between plants and rows, respectively and were evaluated for growth, yield and yield components. The analysis of variance performed for average internodes number, canopy diameter, height up to the primary branch, length of the primary branch, number of branches on the main stem and total height of coffee plant showed significant variations across locations. From the present evaluation, it was noted that the highest Clean Coffee Yield (16.6 and 14.2 quintal per hectare) was scored by coffee cultivars F-59 and 1377A, respectively. Moreover, because of the low quality of coffee delivered to the market, the coffee produced in the Gamo Gofa area (garden coffee) is categorized under forest coffee at the national level. If the quality is improved, the coffee in the area would have its appropriate brand name and the growers and traders can get better returns from their investment. Therefore, those varieties investigated are good candidates for future coffee cup quality analysis.Item Coffee genotypes morphophysiological adaptation under coffee leaf rust biotic stress(Editora UFLA, 2021) Viana, Mariana Thereza Rodrigues; Azevedo, Harianna Paula Alves de; Pereira, Fernanda Aparecida Castro; Carvalho, Milene Alves de Figueiredo; Guimarães, Rubens JoséThe identification of morphophysiological traits responsible for a better plant behavior when infected is useful for cultivar selection, and become crucial for breeding. We investigated the morphophysiological behavior of coffee genotypes before and after inoculation with the pathogen Hemileia vastatrix, causal agent of coffee rust. With multivariate techniques we identified the characteristics that most contribute to total genetic divergence of the geno types. Ten genotypes of Coffea arabica from the Germplasm Bank of Coffee from Minas Gerais were sown in a nursery and then take to a greenhouse with controlled temperature and humidity. After one month of acclimatization, the artificial inoculation with the fungus H. vastatrix was carried out. The anatomical and physiological evaluations were performed 1 day before inoculation and 160 days after inoculation. When the first symptom emerged, plants were evaluated according to a descriptive scale for coffee rust. We observed significant differences in rust severity and ostiole opening between genotypes. Different groups were formed by the K-means method, based on morphophysiological characteristics. This shows that genetic variability exists between the coffee genotypes evaluated before and after inoculation with the pathogen. The most important characteristics that contributed to the total genetic divergence were xylem vessel diameter and stomatal conductance. In conclusion, inoculation with H. vastatrix caused a change in coffee geno types based on morphophysiological characteristics.Item MGS Aranãs: the new Arabica coffee cultivar developed by Epamig with wide adaptation(Editora UFLA, 2021) Botelho, Cesar Elias; Abrahão, Juliana Costa de Rezende; Pereira, Antônio Alves; Oliveira, Antônio Carlos Baião de; Carvalho, Gladyston Rodrigues; Ferreira, André DominghettiThis paper describes the MGS Aranãs cultivar (H32-11-17-4-2-MS-MS) resulting from a cross between the cultivars Icatu 3851-2 and Catimor. The MGS Aranãs cultivar is characterized by small size, ripe red fruits, large seeds, coffee leaf rust resistance, an architecture suitable for densification, production stability, and high beverage quality. This new cultivar is recommended for the Sul de Minas, Cerrado Mineiro and Vale do Jequitinhonha regions.Item Environmental stratification and performance of Coffea canephora clones grown in the Western Amazon(Editora UFLA, 2021) Moraes, Marcos Santana; Rocha, Rodrigo Barros; Ferreira, Fábio Medeiros; Souza, Carolina Augusto de; Espindula, Marcelo Curitiba; Teixeira, Alexsandro LaraChange in the performance of clones grown in different environments is an important question for Coffea canephora breeding. The aim of this study was to evaluate environmental stratification and the performance of C. canephora clones grown in the Western Amazon. For that purpose, the mean yield of three crop seasons was considered to evaluate the performance of 20 genotypes grown in 6 clonal competition trials in the environments of: E1: Ouro Preto do Oeste-RO, E2: Porto Velho-RO, E3: Ariquemes-RO, E4 and E5: Rio Branco-AC and E6: Alta Floresta do Oeste-RO. The trials were conducted with a plant spacing of 3 m × 1.5 m in a complete block experimental design, with three replications of eight plants per plot. Combined analysis indicated significance of the genotype × environment (G×E) interaction and favorable conditions to obtain gains from selection. Reduction in the dimensionality estimated from climate and soil characteristics indicated that the environments of Porto Velho-RO, Rio Branco-AC and Ariquemes-RO are more similar to each other than the environments of Ouro Preto do Oeste-RO and Alta Floresta-RO of greater natural soil fertility and higher altitude. The AMMI1 biplot shows that genotypes 16, 10, and 13 had the highest mean yields, together with greater stability. In the AMMI2 scatterplot (IPCA1×IPCA2), the environ ments E4 and E5 were clustered in the same sector. Clustering based on the complex fraction of the G×E interaction coincided with the AMMI2 scatterplot that clustered the E4 and E5 environments in a single mega-environment. Except for these environments, all the others clustered as locations of different biotic and abiotic stress conditions. This result shows the importance of maintaining evaluations in these environments, which represent the conditions of the coffee fields in the region.Item Identification of acid-tolerant coffee genotypes in a coffee germplasm collection of Colombia(Editora UFLA, 2020) Acuña-Zornosa, Ricardo; Sadeghian-Khalajabadi, SiavoshOne of the limitations of coffee production in many regions of Colombia is the soil acidity. According to historical soil chemical analysis records, more than 50% of coffee farms have pH values below 5.0. Because acid-tolerant coffee varieties are not available, farmers use calcareous additives to correct the problem, which incurs associated labor and input costs. The objective of this work was to identify acid-tolerant genotypes of Coffea arabica. For two contrasting soils in the coffee-growing area of Colombia (Andisol and Entisol), the effect of soil acidity on the growth of 20 genotypes of Coffea Arabica during the seedling stage was evaluated. The genotypes were wild accessions that make up the Colombian Coffee Germplasm Collection and the Castillo ® Naranjal Variety, used as commercial material. Six months after the seedlings were transplanted into soils treated with or without acidity correction additives, the weight of the dry matter of the roots, stems and leaves was recorded. Later, the acid-tolerant genotypes were identified by means of the quadrant method and the tolerance index. The Timor Hybrid and Rume Sudan genotypes were identified as tolerant of the acidity of the two soil types. These genotypes could be used as progenitors in a coffee breeding program leading to a commercial coffee variety tolerant to soil acidity.Item Physiological aspects and yield in coffee progenies with large beans(Editora UFLA, 2021) Pavan, João Paulo Silva; Santos, Cyntia Stephânia dos; Freitas, Ana Flávia de; Carvalho, Samuel Pereira de; Carvalho, Gladyston RodriguesGenetic coffee breeding is aimed at increasing yield associated with tolerance or resistance to biotic and abiotic factors, besides providing a better beverage quality and supplying the demand for bigger beans. The efficiency in photosynthetic activity can limit produce and diversify genotypes, mainly under adverse environmental conditions. Consequently, the importance of selection of Coffea arabica L. regarding these characteristics stands out. Therefore, the objective of this paper was to measure the physiological characterization and yield of Coffea arabica L. progenies with large beans “Big Coffee VL”. Twelve productive progenies were selected and classified according to fruit size (“small”, “medium” and “large”), which were: S14, S23, S34, S36, M4, M5, M14, M20, L10, L12, L17 and L31. Net photosynthetic rate (A), stomatal conductance (gs), transpiration rate (E), water use efficiency (WUE), internal carbon (Ci), intercellular CO2 concentration in the mesophyll were evaluated on the current external CO2 concentration (Ci/Ca), besides vapor pressure deficit (VPD), leaf temperature (Tleaf), indirect determination of the relative levels of chlorophylls a, b and total, in addition to bean yield in two crops. The Tocher grouping resulted in the formation of 4 groups, and progenies M4, L10 and S34 remained in isolated groups.Progeny L10 stood out for higher mean values of A, gs and Ci; the opposite behavior was observed in progeny S34. Progeny M4 is noteworthy as the one with the highest yield in the two years considered, in addition to presenting high photosynthetic rate and chlorophyll indexes. It is concluded that the progenies of “Big Coffee VL” show variability for physiological parameters and productivity. Progenies S14, M4 and L10 stood out, and S14 was more efficient in the use of water; M4 was the most productive and L10 stood out in terms of gas exchange.