Coffee Science
URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/3355
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Resultados da Pesquisa
Item Low concentration fertigation solution allows greater macronutrient use efficiency in coffee seedlings(Editora UFLA, 2021) Rosa, Kelly Martins; Paiva, Paulo Eduardo Branco; Coelho, Victor Peçanha de Miranda; Carvalho, Mychelle; Assis, Henrico Luis Bizão deCoffee seedling production in cone-shaped containers and substrate results in lower sanitary risk and costs. With the use of small containers and sub strates with low fertilizer levels, fertigation is necessary for seedling development. An experiment was carried out with three macronutrient concentra tions (electrical conductivities of 1.0, 2.0 and 3.0 dS m-1) and a non-fertilized control using Coffea arabica cv. Topázio seedlings in 50 cm3 cone-shaped containers and composted pine bark-based substrate. The following fertilizers (expressed in g m-3 of water) were used in the nutrient solution with EC of 1.0 dS m-1: Ca(NO3)2 (400), KNO3 (250), MgSO4 (175), (NH4)PO4 (50) and ConMicros Standard® (25). In the solutions with 2.0 and 3.0 dS m-1, macronutrient concentration was doubled and tripled, while micronutrients remained the same. At four months we assessed EC and pH of the solution drained, shoot dry mass, shoot nutrient concentration and accumulation and macronutrient use efficiency. Fertigation with 1.0 and 2.0 dS m-1 produced seedlings with greater mass (+10%) than fertigation with 3.0 dS m-1, while higher macronutrient supplies increased shoot N, P, K, Mg and S concentration. Fertigation with 2.0 dS m-1 caused greater shoot N, P, K and Ca accumulation and this conductivity also resulted in higher accumulation of B, Fe, Mn and Zn without increasing the micronutrient supply. The response to the increase in Ca in the nutrient solution is evident in the accumulation of this nutrient in the plant but not its concentration, possibly due to the dilution effect. Micronutrient accumulation in this species is also more adequate for determining nutritional status than the concentration in the plant. The least concentrated macronutrient solution increases nutrient use efficiency in C. arabica seedlings as well as being economically and environmentally more sustainable.Item Development of coffee seedlings with biostimulants(Editora UFLA, 2021) Moreira, Wanderson Lopes; Ferraz-Almeida, RiselySustainable coffee production has become an alternative to aggregate value to coffee. Biostimulants are presented to increasing coffee quality and production. This study aims to compare and evaluate the application of biostimulants in the initial development of coffee seedlings. An experiment was carried out with applications of biostimulants: (i) 4-indole-3-ylbutyric acid + gibberellic acid + cinetina, AIA+AG+C; (ii) foliar fertilizer + algae, F+A; (iii) vegetal extracts + gibberellic acid, EV+AA. After 94 days of applying the biostimulants, monitored the length of the aerial part (LAP), the number of leaves (NL), stem diameter, root biomass (pivoting, auxiliary and total). Results showed that the F+A promoted the greatest LAP, NL, and root biomass. AIA+AG+C showed similar performance as F+A in NL. There was a higher production of auxiliary roots with biostimulants impacting positively on plant paraments. Given the results, we concluded that biostimulants based on mixed foliar fertilizer + algae demonstrated to be a better alternative to increasing the devel opment of the aerial and root part of coffee seedlings.Item Propagation of coffee conilon “Vitória” in conventional and alternative substrates(Editora UFLA, 2020) Berilli, Sávio da Silva; Salles, Rodrigo Amaro de; Sales, Ramon Amaro de; Zooca, Alan Alvino Falcão; Ribeiro, Helder Rodrigues; Berilli, Ana Paula Candido Gabriel; Costa, Taiara Souza; Mendes, Tiago Pacheco; Quartezani, Waylson ZancanellaThe sustainable production of inputs or products, either agricultural or industrial, has become an international concern in the last years. Thus, one of the biggest challenges today is to combine production with sustainability. Industrial waste use in agriculture is an alternative that has been gaining strength. The objective of this study was to evaluate the development, physiology and quality of Coffea canephora seedlings from the 13 genotypes (variety Vitória Incaper 8142), in conventional substrate in comparison to a substrate based on tannery sludge. A 2 x 13 factorial scheme was used in which the first factor was the two substrates (conventional substrate and substrate with tannery sludge), and the second factor was the 13 Vitória - Incaper 8142 Conilon coffee genotypes, arranged in a randomized block design with four repetitions. At 150 days after planting, the following characteristics were evaluated: leaf number, plant height, stem diameter, canopy diameter, leaf area, SPAD chlorophyll index, nitrogen balance index, total chlorophyll index, anthocyanins index and flavonoids index, dry shoot matter mass, dry root matter mass,dry total matter mass and Dickson quality index . No significant interaction was observed between the factors evaluated. The substrate containing tannery sludge provided less gain for most of the growth characteristics when compared to the conventional substrate. The tannery substrate promoted a greater production of defense metabolites in plants. As for the response pattern between the genotypes of the Vitória - Incaper 8142 variety, a high unevenness was observed in the initial development, both for their phenological and physiological characteristics, which is a reflect of the genetic variability existing between the genotypes.Item Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds(Editora UFLA, 2020) Oliveira, Leonardo Araujo; Souza, Genaina Aparecida de; Silva, Bruno Tavares; Rocha, Antônio Augusto Gomes; Picoli, Edgard Augusto de Toledo; Pereira, Diego de Sousa; Donzeles, Sérgio Maurício Lopes; Ribeiro, Marcelo de Freitas; Ferreira, Williams Pinto MarquesThe endosperm of coffee beans is an important structure which is composed of different reserve compounds. In the present study, we followed anatomical features during the reserve mobilization of Coffea arabica L. cv ‘Catuaí’ seed samples, harvested at 20 different sites, after 0, 3, 6, 12, 24, 30 and 45 days of imbibition. Seed samples were properly stored for enzyme activity assessment and fixed in FAA solution (formaldehyde, acetic acid and ethanol, 1; 1; 18) and kept in ethanol 70% solution, for histochemical analysis. Alternatively, samples for phenolic compounds histochemical test were fixed in Ferrous sulfate in formalin. Fixed samples were cross-sectioned for detection of starch, lipids, essential oils, oleoresins, proteins, phenolic compounds, neutral polysaccharides, alkaloids, β-1,3 and β-1,4 glucans, and lignin. Overall, based on histochemical tests and enzyme activity, seed reserve mobilization was similar among the evaluated altitudes and mountainsides. During soaking, there is an intense consumption of reserve compounds, starting at the region close to the embryo. Reserve mobilization intensifies after root protrusion, from 12 days of soaking. The intensification of the reserve consumption concomitant with an increased activity of MDH is observed at the first 12 days, whereas SOD showed higher expression after 30 days of soaking. At the 30th day, little reserve is observed in the endosperm. At 45th day, cotyledon leaves are expanded, and the root system, constituted by the taproot and lateral roots, is well established.Item Ultrastructural damage in coffee seeds exposed to drying and to subzero (°C) temperatures(Editora UFLA, 2020) Coelho, Stefânia Vilas Boas; Rosa, Sttela Dellyzete Veiga Franco da; Clemente, Aline da Consolação Sampaio; Lacerda, Laura Nardelli Castanheira; Silva, Luciano Coutinho; Fantazzini, Tatiana Botelho; Ribeiro, Fernando Augusto Sales; Castro, Elisa de MeloDuring drying and freezing, protective mechanisms act to maintain seed physiological quality. Some of these mechanisms contribute to the integrity of cell membranes. The damage caused to cell membranes due to cell stress can be seen in ultrastructural studies, comparing these results to those of physiological evaluation. The aim of this study was to investigate ultrastructural changes in endosperm cells of coffee seeds brought about by drying and by exposure to low temperatures. Seeds of Coffea arabica were dried in silica gel to moisture contents of 40, 20, and 5 % (wb) and brought to equilibrium at temperatures of 10, -20, and -86 oC. Germination, vigor, and tetrazolium tests were performed for evaluation of seed physiological quality. Ultrastructural damage was analyzed by scanning electron microscopy. Coffee seeds with 40% moisture content have whole, swollen, and expanded cells, with a filled lumen and without signs of damage. The physiological and ultrastructural quality of seeds exposed to below zero temperatures with 40% moisture content is compromised. They have null germination and empty cells, indicating leakage of cell content. Drying of coffee seeds leads to uniform contraction of inner cell content. Drying of coffee seeds to 5% moisture content leads to intense contraction of cell volume, with physiological and ultrastructural damage.Item Coffee seedling growthafter legume cultivation in soils with contrasting phosphorus contents(Editora UFLA, 2021) Pereira, Djalma Silva; Costa, Liovando Marciano da; Carmo, Davi Lopes do; Rocha, Ana Caroline Teixeira; Pereira, Antônio AlvesCoffee seedling growth depends on soil phosphorus (P) availability and may be influenced by from pre-cultivation with legumes. Efficient and sustainable ways to increase the bioavailability of P through the recovery of P adsorbed by the soil matrix should be sought. This study proposed to evaluate the growth and P-use efficiency of coffee seedlings cultivated in soils with different P availability after cultivation with legumes. The experiment was carried out in a fully randomized design. Treatments were arranged in a factorial scheme [(2 x 4) + 1]: two soil types, pre-cultivation with four legume species, and one control (without pre-cultivation). The studied soils comprised a Typical Acriferic Red Oxisol (LVwf) with low-P availability and a Typical Chernossolic Litholytic Entisol (RLm) with high-P availability. The legume species Crotalaria juncea, Cajanus cajan, Canavalia ensiformis, and Mucuna aterrima were previously cultivated for 45 days. Afterwards, coffee seedlings were transplanted to the pots, which were then grown for 120 days until evaluations. We assessed the following parameters: plant height (H), stem diameter (SD), shoot dry matter (SDM), root dry matter (RDM), total dry matter (TDM), and shoot contents of macronutrients. Our results showed that coffee seedlings grew more when cultivated in the high-P availability soil, with increments of 13.05% in H, 4.86% in SD, 46.98% in SDM, 17.61% in RDM, and 41.80% in TDM.We also observed an increase of 28.09% in shoot P contents for coffee seedlings grown in RLm. Moreover, pre-cultivation with C. juncea provided the largest increases in coffee seedling growth compared to the control. When grown after C. ensiformis cultivation, coffee seedlings had the highest shoot contents of P, Ca, Mg, and S, which, compared to control, increased by 45%, 39%, 18%, and 17%, respectively.Item Implications of the cut type and apex length of stem cuttings used for the production of plantlets of Conilon coffee(Editora UFLA, 2020) Verdin Filho, Abraão Carlos; Freitas, Silvio de Jesus; Comério, Marcone; Volpi, Paulo Sérgio; Colodetti, Tafarel Victor; Rodrigues, Wagner Nunes; Fonseca, Aymbiré Francisco Almeida da; Posse, Sheila Cristina Pruculi; Fontes, Alexandre Gomes; Christo, Bruno Fardim; Vieira, Luciano Junior DiasProducing plantlets of Conilon coffee within the specific recommendations and with a high level of quality is fundamental since it is capable of promoting the initial development of the crops. To identify the best protocol to prepare the stem cuttings is fundamental to the process of plantlet production of the species. In this context, this study aimed to evaluate the implications of the type of apex cutting and the length of the remaining apex of stem cuttings to produce plantlets of Conilon coffee (Coffea canephora). To this end, two trials were conducted in the Marilândia Experimental Farm (Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural, Marilândia-ES). The first trial evaluated the types of apex cutting (straight or bevel cut), and the second trial studied the different lengths of the remaining apex (0.5, 1.0, 1.5, 2.0, and 2.5 cm). Characteristics of the vegetative growth and photosynthetic traits of the plantlets of Conilon coffee were evaluated after 120 days of cultivation in a nursery. The biomass accumulation of the plantlets of Conilon coffee produced by stem cutting may be favored by the use of bevel cut on the apex. The length of the remaining apex does not seem to have a expressive effect over the quality or growth of the plantlets, being only possible to observe effects for leaf area and biomass accumulation.Item Rooting biostimulants for Coffea arabica L. cuttings(Editora UFLA, 2020) Azevedo, Harianna Paula Alves de; Carvalho, Alex Mendonça de; Vidal, Douglas Alves; Santos, Heloisa Oliveira dos; Doria, JoyceIn the rooting process of Arabica coffee cuttings, plant hormones are used for inducing root formation. However, synthetic molecules cannot be used to form organic seedlings. The objective of this work was to evaluate the effect Cyperus haspan extract and different concentrations and types of humic substances on Arabica coffee cuttings. Arabica coffee shoots were collected, and cuttings containing at least two buds were taken. The experiment had a three-factor design, 2 (presence or absence Cyperus haspan extract) x 2 (humic or fulvic acid) x 4 (0, 10, 25 and 50 mg dm-³ concentrations). The following vegetative data were evaluated: shoot height (cm), survival, remaining leaves, leaf pairs, vigor, number of shoots,root length, area root, volume root, diameter root and fresh and dry mass were also evaluated. The enzyme activity of H+-ATPase, catalase, superoxide dismutase, alcohol dehydrogenase and esterase was also quantified. The results showed that the Arabica coffee cutting rooting rate was increased when treated with humic acid at a concentration of 10 mg dm-³ in the presence of Cyperus haspan extract. The lowest oxidative stress was observed in the 10 mg.dm-³ humic and fulvic acid treatments, regardless of the use of the extract.Item Evaluation of the effect of Azospirillum brasilense inoculation on arabic coffee seedlings(Editora UFLA, 2020) Silva, Leonardo Caixeta da; Barbosa, Camila Karen Reis; Franco Junior, Kleso SilvaIn fabaceae, Azospirilum is used in association with Rhizobium bacteria to promote biological nitrogen fixation, making the plant tolerant to water stress and resulting in better nutrient utilization, obtaining a plant more productive and vigorousand increase roots for better nutrient uptake by the plant. However, there are no studies using coinoculation in Coffee. The objective of the study was to evaluate the effect of different doses of A. brasilense on the development of coffee seedlings. The experiment was carried out in Machado, southern Minas Gerais. The experimental design was completely randomized (DIC), with five treatments and nine replications, totaling 45 experimental plots. The experimental unit consisted of pots of 12 liters of capacity with a substrate composed of 70% soil and 30% of tanned manure and a coffee seedling of cultivar Catuaí Vermelho IAC 144. Treatments were composed with different doses (0, 0.75, 1.5, 2.25 and 3.0 mL) of Azospirillum brasilense inoculant from the Micro-Chemistry laboratory, Azofix®, having Ab-V5 strains, 1 x 108 cfu. mL-1 viable cells per mL. From stage V7, the seedlings received eight applications with a seven-day interval. The applying was done in the late afternoon, under favorable environmental conditions, using a hand sprayer. Evaluations were performed 150 days after the first application. The variables evaluated were shoot height (ALT) (cm); shoot fresh matter (MFPA) (g); root fresh matter (MFR) (g); leaf fresh matter (MFF) (g); stem diameter (DIA)(mm) and pivoting root length (CRP) (cm). It is concluded that the inoculation of coffee seedlings with bacteria of the species Azospirillum brasilense promotes greater growth of the pivoting root as well as the fresh matter of the whole root system of coffee seedlings.Item Impact of drying methods over the germinative potenti al of conilon coffee of late maturation(Editora UFLA, 2019-10) Posse, Sheila Cristina Prucoli; Rodrigues, Wagner Nunes; Comério, Marcone; Volpi, Paulo Sérgio; Verdin Filho, Abraão Carlos; Posse, Robson Prucoli; Oliveira, Vinicius de Souza; Arantes, Sara DousseauThis study aimed to evaluate the effect of different drying methods (regarding temperature and drying time) over the seed moisture and germination of seeds of Coffea canephora, harvested from genotypes of late maturation. For this end, seeds from selected genotypes were harvested and dried using two different methods: drying at 25 ˚C with natural circulation of air and drying at 35 ˚C with artificial circulation of air. The seeds were evaluated to quantify the loss of moisture content, and their germinative potential was assessed by parameters of germination speed, and the proportion of seedlings with healthy germination originated from them. The results show it is possible to apply different drying methods; using different systems, temperatures and times of exposure; to decrease the moisture content of seeds of C. canephora, from 50% (freshly harvested seeds from genotypes of late maturation, in July) to under 18% (dried seeds). Even if the initial stages of the drying process, while the seeds still present high moisture content, are similar for both methods, the method with artificial circulation of air at 35 ˚C promotes the drying speed and is able to achieve the lower levels of seed moisture using less than half of the time required by method with natural circulation of air at 25 ˚C. This faster moisture removal, however, has negative impacts over the germinative potential of the seeds, which are not observed for seeds subjected to the slow natural drying.