Coffee Science - v.14, n.3, 2019
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/12417
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Resultados da Pesquisa
Item Evaluation of probiotic microencapsulation in a prebiotic mixture with coffee extract(Editora UFLA, 2019-07) Zapata, Angela María Ormaza; Arango, Félix Octavio Díaz; Rodríguez-Barona, SneyderThe objective in the present study was to evaluate coffee extract as an encapsulant material, when mixed with prebiotic carbohydrates, in order to obtain a coffee-based, non-dairy probiotic beverage. Six coffee extract formulations were evaluated with maltodextrin and inulin, in various proportions, for the obtaining of microcapsules with the probiotic strain L. casei ATCC 393, by way of spray drying. The best formulations encountered were MD-INU:EXT (50:50) and EXT:Water (50:50), which were spray dried, for increased viability, elevated wettability and solubility, low hygroscopicity, and adequate morphology for this type of powdered product. The formulation was composed of maltodextrin, inulin, and coffee extract, and MD-INU:EXT (50:50) presented the best physicochemical and viability characteristics. The potential of coffee extract was proven as a wall material in microencapsulated, non-dairy, highly acidic, probiotic products. Optimal viability was achieved, in the order of 1x106 CFU/mL, for both selected formulations, which maintained functional potential for up to 11 weeks of storage.Item Selection of fungal isolates with potential for phosphate solubilation and formulation of inoculant for coffee crops(Editora UFLA, 2019-07) Chalfoun, Sara Maria; Angélico, Caroline Lima; Resende, Mário Lúcio Vilela de; Moraes, Graziella Evaristo deThe dependence on chemical fertilizers for the future of agriculture will result in additional soil health losses, possibility of water contamination and continued currency avoidance due to the increasing dependence on imports. The indiscriminate use of synthetic fertilizers has polluted the soil and watersheds, destroying useful microorganisms and insects, what makes crops more susceptible to diseases. Considering this critical scenario, microorganisms have stood out as a potential alternative for maintaining the productivity, reliability and sustainability of the global food chain. In order to increase the productivity and/or reduce the amount of fertilizers, as well as to meet the requirements for the use of low-solubility phosphorus sources, microorganisms were isolated from the rhizosphere and artificially cultivated. Subsequently, they were selected regarding their potential for solubilizing phosphorus and incorporated into a low-cost matrix. A microbial inoculant was thus developed and tested in a coffee crop. It was possible to formulate an inoculant based on an isolate selected from Aspergillus niger, using an inactivated seed and sodium alginate as matrices. It was also observed that there was a greater correlation between the evaluated parameters and increasing doses of the inoculants when Araxá apatite was used as source of phosphorus, proving the importance of the inoculants as release factors and availability of phosphorus in the case of sources less soluble, giving Brazilian agriculture greater autonomy in the input market.Item Spatial-temporal patterns of coffee tree physiology(Editora UFLA, 2019-07) Santini, Paula Tristão; Barbosa, Ronei Aparecido; Almeida, Lorena Gabriela; Souza, Kamila Rezende Dázio de; Barbosa, João Paulo Rodrigues Alves Delfino; Alves, José DonizetiThe ecophysiological parameters of coffee canopy were mapped throughout the day. Therefore, evaluations were carried out in ‘Catuaí Vermelho’ Coffea arabica L., measuring 1.7 meters. A vertical gradient (from the apex to the base of the plant canopy) and a horizontal gradient (plagiotropic branches) were established to analyze different positions of the canopy. Thus, in the vertical direction, four heights were analyzed in the plant: top, upper, middle and lower regions. In the horizontal gradient, the plagiotropic branches were divided into three parts: basal, median and apical. Collection was performed on the east and west sides of the canopy, at four times of the day: 6 a.m., 9 a.m., noon, and 3 p.m., totaling 24 collection points at each time. Gas exchange, photosynthetically active radiation and leaf temperature were evaluated in each of the 24 points of the coffee canopy. The gas exchange characteristics of an individual coffee leaf diverge considerably from other leaves, which require caution when scaling estimates of leaf photosynthesis at the global canopy level. The analysis of some punctual leaves does not serve to discriminate the overall dynamics of a canopy.Item Sites and sampling tools in the soil fertility evaluation of coffee plants in production(Editora UFLA, 2019-07) Mantovani, José Ricardo; Lima, José Antônio; Garcia, Ciro Pozzi; Rezende, Tiago TeruelIn soil sampling, the collection site and the tool used may induce errors that can compromise the results of the chemical analysis and, consequently, the definition of corrective and fertilizer doses. The objective in this study was to evaluate soil sampling sites and tools in a coffee growing area. The experiment was carried out in a field in the municipality of Alfenas-MG, in a 35-year coffee field. A randomized block design was used in a 3 x 5 factorial scheme and 4 replications, totaling 60 experimental units. The treatments consisted of 3 sampling sites: in the fertilizer strip (located in the projection of the plant canopy); between rows; and in the total area, with 1/3 of the samples collected under the canopy and close to the plant stem, 1/3 in the canopy projection and 1/3 collected between rows; and 5 sampling tools: Dutch auger; screw auger; probe; hoe and drill. Soil samples were collected at a depth of 0 to 0.2 m, and each composite sample was obtained by assembling and mixing 20 single samples. At each sampling point, an area of 2m 2 was delimited, in which simple samples were collected in all the evaluated places and with all the tools used. The following parameters were determined in the soil samples: pH in CaCl 2 ; potential acidity (H+Al); organic matter; P, extracted by Mehlich; K+, Ca2+, Mg2+ and base saturation. In a coffee-growing area, the fertilizer strip (canopy projection) has topsoil with higher acidity and lower contents of organic matter and nutrients P, K+, Ca2+ and Mg2+ than between rows. The tools used for soil sampling do not influence the results of soil chemical analysis in the coffee-growing area.Item Sensory profile of arabica coffee accesses of the germplasm collection of Minas Gerais – Brazil(Editora UFLA, 2019-07) Fassio, Larissa de Oliveira; Pereira, Rosemary Gualberto Fonseca Alvarenga; Malta, Marcelo Ribeiro; Liska, Gilberto Rodrigues; Sousa, Maísa Mancini Matioli; Carvalho, Gladyston Rodrigues; Pereira, Antônio AlvesThis study was developed to determine the beverage quality and the sensory profile of Coffea arabica accesses of the Germplasm Collection of Minas Gerais State in two consecutive harvests. Mature coffee fruits from 49 accesses were selectively harvested in the 2015 and 2016 harvests, and after processing and drying they were sensorially evaluated by a team of trained judges in accordance to the protocols of the Specialty Coffee Association. The data were analyzed by Principal Component Analysis, Cluster Hierarchical Analysis, and Content Analysis. The year of harvest influences the scores of the sensory attributes of the beverage of some coffee accesses. The access 27 (Pacamara) stood out with the highest sensory score seen in the first year of harvest. The accesses 36 (Timor Hybrid UFV376-52) and 43 (BE5 WushWush) stood out for the final sensory score in the two years of evaluation. All accesses belonging to the Timor Hybrid Germplasm stood out in the beverage quality in the second year of harvest. A total of 139 sensory attribute descriptors were identified, and the content analysis showed different levels for the frequency of terms in each access group formed as a function of quality. The Germplasm Collection of Minas Gerais has a wide variability for the production of superior quality coffees, and the year of harvest influences to a greater or lesser degree the potential of quality of the accesses.Item Indaziflam application in newly transplanted arabica coffee seedlings(Editora UFLA, 2019-07) Gomes, Carolina Alves; Pucci, Laís Franchini; Alves, Deivide Patrik; Leandro, Vitor Alves; Pereira, Gustavo Antônio Mendes; Reis, Marcelo Rodrigues dosWeed management is one of the main and most problematic cultural practices used in coffee plantations. Slow growth, broad spacing associated with scarce registered products for weed control at an early crop stage, are the main problems faced by producers. Therefore, it is desirable for new herbicide molecules to be registered for this crop, as is the recent case of indaziflam. This study aimed to know the influence of indaziflam on the morphological characteristics of coffee plants and to propose alternatives to reduce its effects on plants. Therefore, the effects of this herbicide on two arabica coffee cultivars were studied, as well as the association of this herbicide with protection of the seedling collar (PVC pipes and bovine manure). Controls on weeds, possible injury on seedlings and effects on the main morphological characteristics of coffee plants were evaluated. Although treatments with the use of indaziflam cause intoxication in the seedlings, a reduction in plant damage was observed over time, and the damage was insignificant. In general, it was observed that cultivar IBC 12 suffered minor injuries, but it is more sensitive to the presence of indaziflam, since it presented greater damage in the morphological variables. The use of manure with the PVC pipe can be an alternative to reduce the effects of this herbicide, as it reduced the effects of the product on the root system of this cultivar. It is concluded that the application of indaziflam causes mild symptoms in coffee plants independent of the planted cultivar. Indaziflam causes reduction in seedling morphology, and this effect is dependent on the cultivar used. The use of manure associated to the PVC pipe can be an alternative to reduce the effects of indaziflam, mainly in the root system of coffee plants.Item Spatio-temporal variability of carbohydrate and chlorophyll content in the coffee canopy(Editora UFLA, 2019-07) Santini, Paula Tristão; Almeida, Lorena Gabriela; Souza, Kamila Rezende Dázio de; Barbosa, João Paulo Rodrigues Alves Delfino; Alves, José DonizetiThe spatial variability of the total chlorophyll content and carotenoids content, starch and soluble sugars of coffee canopy were mapped throughout the day. Therefore, evaluations were carried out in a ‘Catuaí Vermelho’ coffee plant with 1.7 meters height. A vertical gradient (from the apex to the base of the plant canopy) and a horizontal gradient (plagiotropic branches) were established to analyze different positions of the canopy. Thus, in the vertical direction, four heights were analyzed in the plant: top, upper, middle and lower regions. In the horizontal gradient, the plagiotropic branches were divided into three parts: basal, median and apical. Collection of leaf samples was performed on the east and west sides of the canopy, at 9 a.m., totaling 24 collection points at each time. Higher content of photosynthetic pigments and concentration of sugars were observed in the western face and in the inner parts of the coffee tree. The content of chloroplast pigments and sugars of an individual coffee leaf diverge considerably from other leaves, which requires caution when scaling estimates at the global canopy level. The analysis of some punctual leaves does not serve to discriminate the overall dynamics of a canopy.Item Association of indaziflam and oxyfluorfen in coffee pre-transplantation(Editora UFLA, 2019-07) Pucci, Laís Franchini; Fukada, Paulo Massaharu; Pereira, Gustavo Antônio Mendes; Alves, Deivide Patrik; Gomes, Carolina Alves; Reis, Marcelo Rodrigues dosWeed management in the pre-transplantation of coffee plants is an essential condition for the future success of the crop. Currently, the producer has few options to perform chemical management at this stage. Therefore, one of the solutions that is being used is the tank mixing of products proven efficient with new products on the market, such as indaziflam. The objective of this study is to know the selectivity of indaziflam, associated or not with oxyfluorfen, in coffee pre-planting and its effectiveness in weed control. Thus, the following treatments were tested in the field: indaziflam (25 g ha -1 a.i.), indaziflam (50 g ha -1 a.i.), oxyfluorfen (960 g ha -1 a.i.), indaziflam (25 g ha -1 a.i.) + oxyfluorfen (960 g ha -1 a.i.), indaziflam (50 g ha -1 a.i.) + oxyfluorfen (960 g ha -1 a.i.), plus a weeded and a non-weeded control. All treatments caused low intoxication in the seedlings, but without impairing the development and morphological variables of the plants. The treatments were efficient in controlling the species Bidens pilosa L., Commelina benghalensis L., Urochloa decumbens, Emilia fosbergii and Melampodium perfoliatum until 68 days after application. At 104 days, it was observed that there was a reduction in the efficiency of the products. It is concluded that the associated use of indaziflam and oxyfluorfen does not cause intoxications capable of interfering with the development of coffee plants. The association of these herbicides or their isolated applications is efficient in the control of weeds in the pre-transplantation of coffee plants, and the choice of the best management strategy is dependent on the weed community present in each area.Item Arabica coffee cultivars in different water regimes in the central cerrado region(Editora UFLA, 2019-07) Veiga, Adriano Delly; Rodrigues, Gustavo Costa; Rocha, Omar Cruz; Bartholo, Gabriel Ferreira; Guerra, Antônio Fernando; Silva, Thiago Paulo daPhenotypic characterization of coffee cultivars under an irrigation system, as well as adaptability to controlled water stress, aiming at flowering uniformity, high yield and grain quality, plays an important role in coffee production in the cerrado areas. A field trial was carried out aiming to evaluate the agronomic performance of arabica coffee cultivars under different water regimes, using center pivot irrigation: irrigation throughout the year (WR1); suspended at the end of June for 40 days until leaf water potential reached -1.5 MPa (WR2); suspended at the end of June for 70 days until leaf water potential reached -2.3 MPa (WR3); suspended at the end of June for 100 days until leaf water potential reached -3.4 MPa (WR4); and a non-irrigated regime (WR5). The following traits were analyzed: plant height, stem diameter, canopy projection, number of plagiotropic branches, coffee grain yield, percentage of fruit in the cherry stage, and sieve retention percentages. Higher yield, plant growth, and percentage of fruit in the cherry stage are observed in the water regime with seventy days of controlled water stress (WR3). The Obatã IAC 1669-20 cultivar exhibits high yield and plant growth values in an irrigated system, and Catuaí Amarelo IAC 86 stands out in the non-irrigated system. For these genotypes, the coffee grain yield is most highly correlated with number of reproductive branches.Item Initial stages of infectious process of Pseudomonas syringae pv. garcae in coffee leaves(Editora UFLA, 2019-07) Souza, José Otávio Gusmão de; Pozza, Edson Ampélio; Belan, Leônidas Leoni; Freitas, Marcelo Loran de Oliveira; Oliveira, Júlia MarquesBacterial blight of coffee (Pseudomonas syringae pv. garcae) (PSG) has been causing damage and losses to coffee growing. However, initial stages of the infectious process should be described in order to understand the relationship cycle and to propose efficient management strategies. This study described the initial stages of infectious process of the PSG in coffee leaves over time. The bacterial penetration sites studied were stomata, leaf margins, and wounds. Coffee seedlings received 50μL inoculum suspension at marked points on the abaxial face and leaf margins, and around wounds. By 30 minutes, 1, 3, 6, and 12 hours after inoculation, leaf disks collected at inoculated points were analyzed with scanning electron microscopy. One hour after inoculation, bacterial groups could be seen as result of cell multiplication. Subsequently, the number of grouped bacteria increased proportionally over time. A growing population concentration was found near stomata, wounds, epidermal appendages, and wax layer on leaf margins and leaf tip. From these places bacteria were positioned in their long axis starting from structures, thus created a population gradient and displacement flow toward penetration sites. Initial stages of infectious process occurred in all penetration sites, thus it was not possible to determine a preferred pathway.