Comparison of high performance liquid chromatography with fluorescence detector and with tandem mass spectrometry methods for detection and quantification of ochratoxin a in green and roasted coffee beans
Data
2013-11
Autores
Bandeira, Raquel Duarte da Costa Cunha
Uekane, Thais Matsue
Cunha, Carolina Passos da
Rodrigues, Janaina Marques
Cruz, Marcus Henrique Campino de la
Godoy, Ronoel Luiz de Oliveira
Fioravante, Andreia de Lima
Título da Revista
ISSN da Revista
Título de Volume
Editor
Instituto de Tecnologia do Paraná - Tecpar
Resumo
Two analytical methods for the determination and confirmation of ochratoxin A (OTA) in green and roasted coffee samples were compared. Sample extraction and clean-up were based on liquid–liquid phase extraction and immunoaffinity column. The detection of OTA was carried out with the high performance liquid chromatography (HPLC) combined either with fluorescence detection (FLD), or positive electrospray ionization (ESI+) coupled with tandem mass spectrometry (MS–MS). The results obtained with the LC-ESI-MS/MS were specific and more sensitive, with the advantages in terms of unambiguous analyte identification, when compared with the HPLC-FLD.
Descrição
Palavras-chave
HPLC-FLD, LC-MS/MS, Green Coffee, Roasted Coffee
Citação
BANDEIRA, R. D. C. C. et al. Comparison of high performance liquid chromatography with fluorescence detector and with tandem mass spectrometry methods for detection and quantification of ochratoxin a in green and roasted coffee beans. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 6, p. 911-920, nov./dez. 2013.