Scientia Agrícola
URI permanente para esta coleçãohttps://thoth.dti.ufv.br/handle/123456789/12094
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Item Host colonization differences between citrus and coffee isolates of xylella fastidiosa in reciprocal inoculation(Escola Superior de Agricultura "Luiz de Queiroz", 2008-05) Prado, Simone de Souza; Lopes, João Roberto Spotti; Demétrio, Clarice Garcia Borges; Borgatto, Adriano Ferreti; Almeida, Rodrigo Piacentini Paes deCitrus variegated chlorosis (CVC) and coffee stem atrophy (CSA) are important diseases in Brazil associated with closely-related strains of Xylella fastidiosa, but little is know about host overlapping and importance of citrus and coffee as inoculum sources of these strains. In this study, reciprocal-inoculation experiments were performed to determine if CVC and CSA isolates are biologically similar within citrus and coffee plants. These two hosts were mechanically inoculated with a CVC and a CSA isolate of X. fastidiosa at four concentrations ranging between10 3 and 10 9 colony forming units CFU mL -1 . At two, four and eight months after inoculation, the infection efficiency and bacterial populations of the isolates in each host were determined by culturing. The CVC isolate infected both citrus and coffee plants, but developed lower populations in coffee. The CSA isolate did not colonize citrus. Inoculation of coffee plants with the CVC isolate resulted in low rates of infection and required an inoculum concentration ten-fold higher than that necessary to obtain a similar (25%) rate of infection in citrus. The relatively low infection rates and bacterial numbers of the CVC isolate in coffee plants compared with those observed in citrus suggest that coffee is not a suitable host to serve as a source of inoculum of the CVC strain for primary spread to citrus or within coffee plantations. Key words: citrus variegated chlorosis, coffee leaf scorch, inoculum concentration, bacterial population, epidemiologyItem Growth, development, and fertilizer-15N recovery by the coffee plant(Escola Superior de Agricultura "Luiz de Queiroz", 2007-09) Fenilli, Tatiele Anete Bergamo; Dourado-Neto, Durval; Trivelin, Paulo César Ocheuze; Favarin, José Laercio; Costa, Flávio Murilo Pereira da; Bacchi, Osny Oliveira SantosThe relationship between growth and fertilizer nitrogen recovery by perenial crops such as coffee is poorly understood and improved understanding of such relations is important for the establishment of rational crop management practices. In order to characterize the growth of a typical coffee crop in Brazil and quantify the recovery of 15 N labeled ammonium sulfate, and improve information for fertilizer management practices this study presents results for two consecutive cropping years, fertilized with 280 and 350 kg ha -1 of N, respectively, applied in four splittings, using five replicates. Shoot dry matter accumulation was evaluated every 60 days, separating plants into branches, leaves and fruits. Labeled sub-plots were used to evaluate N-total and 15 N abundance by mass spectrometry. During the first year the aerial part reached a recovery of 71% of the fertilizer N applied up to February, but this value was reduced to 34% at harvest and 19% at the beginning of the next flowering period due to leaf fall and fruit export. For the second year the aerial part absorbed 36% of the fertilizer N up to March, 47% up to harvest and 19% up to the beginning of the next flowering period. The splitting into four applications of the used fertilizer rates was adequate for the requirements of the crop at these growth stages of the coffee crop.Item Spatio-temporal modelling of coffee berry borer infestation patterns accounting for inflation of zeroes and missing values(Escola Superior de Agricultura "Luiz de Queiroz", 2009-01) Cárdenas, Ramiro Ruiz; Assunçã, Renato Martins; Demétrio, Clarice Garcia BorgesThe study of pest distributions in space and time in agricultural systems provides important information for the optimization of integrated pest management programs and for the planning of experiments. Two statistical problems commonly associated to the space-time modelling of data that hinder its implementation are the excess of zero counts and the presence of missing values due to the adopted sampling scheme. These problems are considered in the present article. Data of coffee berry borer infestation collected under Colombian field conditions are used to study the spatio-temporal evolution of the pest infestation. The dispersion of the pest starting from initial focuses of infestation was modelled considering linear and quadratic infestation growth trends as well as different combinations of random effects representing both spatially and not spatially structured variability. The analysis was accomplished under a hierarchical Bayesian approach. The missing values were dealt with by means of multiple imputation. Additionally, a mixture model was proposed to take into account the excess of zeroes in the beginning of the infestation. In general, quadratic models had a better fit than linear models. The use of spatially structured parameters also allowed a clearer identification of the temporal increase or decrease of infestation patterns. However, neither of the space-time models based on standard distributions was able to properly describe the excess of zero counts in the beginning of the infestation. This overdispersed pattern was correctly modelled by the mixture space-time models, which had a better performance than their counterpart without a mixture component.Item Comparison between climatological and field water balances for a coffee crop(Escola Superior de Agricultura "Luiz de Queiroz", 2007-05) Bruno, Isabeli Pereira; Silva, Adriana Lúcia da; Reichardt, Klaus; Dourado-Neto, Durval; Bacchi, Osny Oliveira Santos; Volpe, Clóvis AlbertoThe use of climatological water balances in substitution to complete water balances directly measured in the field allows a more practical crop management, since the climatological water balances are based on data monitored as a routine. This study makes a comparison between these methods in terms of estimatives of evapotranspiration, soil water storage, soil available water, runoff losses, and drainage below root zone, during a two year period, taking as an example a coffee crop of the variety Catuaí, three to five years old. Climatological water balances based on the estimation of the evapotranspiration through the methods of Thornthwaite and Penman-Monteith, can reasonably substitute field measured balances, however underestimating the above mentioned variables.Item Spatial variability of leaf wetness duration in cotton, coffee and banana crop canopies(Escola Superior de Agricultura "Luiz de Queiroz", 2008-12) Santos, Eduardo Alvarez; Sentelhas, Paulo Cesar; Pezzopane, José Eduardo Macedo; Angelocci, Luiz Roberto; Monteiro, José Eduardo Boffino AlmeidaDespite the importance of leaf wetness duration for plant disease epidemiology, there has been little attention paid to research on how its variability relates to different cropping situations. The objective of this study was to evaluate the spatial variability of leaf wetness duration (LWD) in three crops, comparing these measurements with turfgrass LWD, obtained in a standard weather station. LWD was measured by electronic sensors in three crops with different canopy structures and leaf area: cotton, coffee and banana. For the cotton crop, cylindrical sensors were deployed at the lower third and on the top of the canopy, facing southwest. For the coffee crop, flat plate sensors were installed in the lower third of the canopy facing northeast and southwest; in the middle third facing northeast and southwest; and inside and on the top of the canopy. For the banana canopy, cylindrical sensors were used to measure LWD in the lower third of the canopy and in the upper third of the plant. Turfgrass LWD was simultaneously measured in a nearby standard weather station. The LWD showed different patterns of variation in the three crop canopies. For coffee plants, the longest LWD was found in the lower portions of the canopy; for the banana crop, the upper third of the canopy showed the longest LWD; whereas for the cotton crop no difference was observed between the top and lower third of the canopy. Turfgrass LWD presented a good relationship with LWD measured on the top or in the upper third of the crops. Thus, the estimate of crop LWD can be perfomed based on turfgrass LWD, this being a useful tool for plant disease management purposes for crops in which the longer LWD occurs at the upper canopy portion.Item Total phenol concentrations in coffee tree leaves during fruit development(Escola Superior de Agricultura "Luiz de Queiroz", 2008-07) Salgado, Paula Rodrigues; Favarin, José Laércio; Leandro, Roseli Aparecida; Lima Filho, Oscar Fontão deVegetables have a natural defense against external factors synthesizing phenolic compounds, which depends on the maturity stage and on the climate. Total phenol grades were extracted from mature and young coffee leaves and were analyzed in relation to yield, phenology and climate. The climatic conditions were described by air temperature, global radiation and daily insolation. Evaluations were made on height, diameter and length of reproductive branches to determine the respective vegetative growth rates of the plants. The amounts of total phenols in the plants at the production stage was 174.0 mg g -1 and 138.9 mg g -1 for young and mature leaves, respectively, and for plants without fruit formation 186.5 mg g -1 and 127.6 mg g -1 for young and mature leaves, respectively. The total phenol concentrations in young leaves with and without fruit formation were 25% and 46% greater compared to mature leaves. The secondary phenol synthesis in seed (146.8 mg g -1 ) was 31% lower than during grain maturation (212.4 mg g -1 ). The total phenol metabolization depends indirectly on the temperature and on the global radiation, presenting an inverse trend in relation to these climatic variables. Crop protection management should take into consideration periods of endanger of this natural defense of the plant.Item Variability of water balance components in a coffee crop in Brazil(Escola Superior de Agricultura "Luiz de Queiroz", 2006-03) Silva, Adriana Lúcia da; Roveratti, Renato; Reichardt, Klaus; Bacchi, Osny Oliveira Santos; Timm, Luis Carlos; Bruno, Isabeli Pereira; Oliveira, Julio César Martins; Dourado Neto, DurvalEstablishing field water balances is difficult and costly, the variability of their components being the major problem to obtain reliable results. This component variability is presented herein for a coffee crop grown in the Southern Hemisphere, on a tropical soil with 10% slope. It was observed that: rainfall has to be measured with an appropriate number of replicates; irrigation can introduce great variability into calculations; evapotranspiration, calculated as a remainder of the water balance equation, has exceedingly high coefficients of variation; the soil water storage component is the major contributor in error propagation calculations to estimate evapotranspiration; and that runoff can be satisfactorily controlled on the 10% slope through crop management practices.Item Bioprospecting endophytic bacteria for biological control of coffee leaf rust(Escola Superior de Agricultura "Luiz de Queiroz", 2006-01) Shiomi, Humberto Franco; Silva, Harllen Sandro Alves; Melo, Itamar Soares de; Nunes, Flávia Vieira; Bettiol, WagnerSuppression of plant diseases due to the action of endophytic microorganisms has been demonstrated in several pathosystems. Experiments under controlled conditions involving endophytic bacteria isolated from leaves and branches of Coffea arabica L and Coffea robusta L were conducted with the objective of evaluating the inhibition of germination of Hemileia vastatrix Berk. & Br., race II, urediniospores and the control of coffee leaf rust development in tests with leaf discs, detached leaves, and on potted seedling of cv. Mundo Novo. The endophytic bacterial isolates tested proved to be effective in inhibiting urediniospore germination and/or rust development, with values above 50%, although the results obtained in urediniospore germination tests were inferior to the treatment with fungicide propiconazole. Endophytic isolates TG4-Ia, TF2-IIc, TF9-Ia, TG11-IIa, and TF7-IIa, demonstrated better coffee leaf rust control in leaf discs, detached leaves, and coffee plant tests. The endophytic isolates TG4-Ia and TF9-Ia were identified as Bacillus lentimorbus Dutky and Bacillus cereus Frank. & Frank., respectively. Some endophytic bacterial isolates were effective in controlling the coffee leaf rust, although some increased the severity of the disease. Even though a relatively small number of endophytic bacteria were tested, promising results were obtained regarding the efficiency of coffee leaf rust biocontrol. These selected agents appears to be an alternative for future replacement of chemical fungicide.Item Performance of Holstein-Zebu cows under partial replacement of corn by coffee hulls(Escola Superior de Agricultura "Luiz de Queiroz", 2005-03) Tavares, Anamaria Almeida Costa; Pereira, Marcos Neves; Tavares, Marcelo Rodrigo; Chaves, Márcio LuizReplacement of corn by citrus pulp or coffee hulls explores the potential of dairy cows to digest fiber-rich feedstuffs. However, for the State of Minas Gerais, Brazil, replacing citrus pulp by coffee hulls may reduce milk production costs, since citrus pulp needs to be imported from another state, while coffee hulls are highly available at essentially no cost. The objective of this experiment was to evaluate the performance of crossbred cows fed concentrates containing 25% coffee hulls (Coffee) or 25% citrus pulp (Pulp) as a replacement for ground corn grain (Corn; 47.4% in the concentrate). Forty-two cows were blocked based on milk yield and allocated to one of the three treatments for 56 days, following a 14-day standardization period. Concentrates were fed twice a day during the milking routine, 1 kg to each 2.5 kg of the average milk yield for the block, determined at the end of the standardization period. The daily 3.5% fat-corrected milk yield was 7.5 kg for Corn, 7.9 kg for Pulp and 6.2 kg for Coffee (P < 0.01). Cows consuming coffee hulls had greater body weight loss (P = 0.03). Feed orts during milking amounted to 30% of the concentrate offered in Coffee, greater than the other treatments throughout the experimental period (P < 0.01). The mean daily intake of coffee hulls was 575 grams. The frequency of cows reluctant to enter the milking parlor yoke was 10% for Coffee and 1% for the other two treatments (P < 0.01). There was no detectable effect of coffee hulls on cow temperament and frequency of defecation during milking, as well as on somatic cell count (P > 0.26). The Pulp concentrate provided the highest financial efficiency, while Coffee was the least efficient (P < 0.01). These data suggest that the potential of use of coffee hulls is low when added at rates equal to or greater than 25% of the concentrate offered during the milking routine.Item Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants(Escola Superior de Agricultura "Luiz de Queiroz", 2007-09) Miranda, Vicente Savonitti; Farias, Paulo Roberto Silva; Roberto, Sérgio Rufo; Lacava, Pedro MagalhãesThe Citrus Variegated Chlorosis and the Coffee Leaf Scorch are some of the many destructive diseases caused by Xylella fastidiosa, a gram-negative bacterium limited to the xylem of affected plants. As its genetic characterization is still not well established, different isolates of X. fastidiosa from citrus and coffee were evaluated through RAPD (Random Amplified Polymorphic DNA) technique to characterize and classify these isolates based on similarity coefficients. Sixteen isolates of X. fastidiosa were used on this trial, obtained from citrus, coffee and almond. The genetic polymorphism evaluation was performed using six arbitrary 10-base primer pairs. It was possible to establish a dendogram in which the isolates were classified into five groups (A, B, C, D and E). A prevalence of citrus isolates in groups A and D was observed. In groups B and C, there was a prevalence of coffee isolates meanwhile the group D consisted of the almond isolate, solely.