Biblioteca do Café
URI permanente desta comunidadehttps://thoth.dti.ufv.br/handle/123456789/1
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Resultados da Pesquisa
Item Checagem da pureza genética de amostras de café Bourbon por meio de marcador microssatélite(Embrapa Café, 2022-11) Padilha, Lilian; Martinati, Juliana Camargo; Guerreiro Filho, Oliveiro; Pereira, Ligia Alves; Maluf, Mirian PerezItem Anatomical changes on coffee leaves infected by Pseudomonas syringae pv. garcae(Grupo Paulista de Fitopatologia, 2015) Rodrigues, Lucas Mateus Rivero; Queiroz-Voltan, Rachel Benetti; Guerreiro Filho, OliveiroAlthough poorly studied, the bacterial halo blight is an important disease in the major coffee-producing states of Brazil. External damage and anatomical changes on leaves were measured in seedlings of Coffea arabica cv. Mundo Novo, susceptible to Pseudomonas syringae pv. garcae, by using histological sections obtained at 10 and 20 days after inoculation (DAI). The changes on the epidermis were smaller than the lesions measured in the mesophyll, irrespective of the evaluated colonization period, showing that the internal damage caused by the bacterium represent twice the damage observed externally. From the inoculation site, lysis occurred on the epidermal cells and on the palisade and spongy parenchyma cells, with strong staining of their cellular contents, as well as abnormal intercellular spaces in the palisade parenchyma, hypertrophy and hyperplasia of mesophyll cells and partial destruction of chloroplasts. Additionally, this study revealed the presence of inclusion bodies in epidermal and mesophyll cells. Bacterial masses were found in the apoplast between and within mesophyll cells. Bacteria were also observed in the bundle sheath and vascular bundles and were more pronounced at 20 DAI, not only near the inoculation site but also in distant areas, suggesting displacement through the vascular system. These results can be useful to understand this plant-pathogen interaction.