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Item Resistance to bacterial halo blight in Arabica coffee lines derivative from the genotype C1195-5-6-2 under natural infection conditions(Crop Breeding and Applied Biotechnology, 2018) Andreazi, Elder; Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Shigueoka, Luciana Harumi; Santos, Willian Gabriel dos; Pereira, Carlos Theodoro MottaThe aim of this study was to identify resistance to bacterial halo blight in Arabica coffee lines carrying Coffea racemosa genes. Eighteen Arabica coffee lines derivative from the genotype C1195-5-6-2, and the cultivars IAPAR 59 and IPR 99 were evaluated for resistance to bacterial halo blight in two trials carried out in field conditions, in Londrina, PR, Brazil. The cultivars Mundo Novo IAC 376-4 and Catuaí Vermelho IAC 81 were included as susceptible controls. Ten lines and the cultivar IAPAR 59 showed resistance to bacterial halo blight. The cultivar IPR 99 presented intermediate reaction, and the controls were very susceptible. This is the first study to show that lines derivative from the genotype C1195-5-6-2, which has C. racemosa genes, could be a source of resistance to bacterial halo blight in coffee breeding programs.Item Genetic polymorphism among 14 elite Coffea arabica L. cultivars using RAPD markers associated with restriction digestion(Sociedade Brasileira de Genética, 2003) Sera, Tumoru; Ruas, Paulo Maurício; Ruas, Claudete de Fátima; Diniz, Leandro Eugênio Cardamone; Carvalho, Valdemar de Paula; Rampim, Leandro; Ruas, Eduardo Augusto; Silveira, Sheila Recepute daKnowledge of the genetic variability among genotypes is important for the transfer of useful genes and to maximize the use of available germplasm resources. This study was carried out to assess the genetic variability of 14 elite Coffea arabica cultivars using random amplified polymorphic DNA (RAPD) associated with a prior digestion of genomic DNA with restriction endonucleases. The accessions were obtained from the Coffea collection maintained at the Instituto Agronômico do Paraná (IAPAR), located in Londrina, Paraná, Brazil. Twenty-four informative RAPD primers, used in association with restriction enzymes, yielded 330 reproducible and scorable DNA bands, of which 224 (68%) were polymorphic. The amplified products were used to estimate the genetic variability using Dice’s similarity coefficient. The data matrix was converted to a dendrogram and a three-dimensional plot using principal coordinate analysis. The accessions studied were separated into clusters in a manner that was consistent with the known pedigree. The associations obtained in the dendrogram and in the principal coordinate analysis plot suggest the probable origin of the Kattimor cultivar. The RAPD technique associated with restriction digestion was proved to be a useful tool for genetic characterization of C. arabica genotypes making an important contribution to the application of molecular markers to coffee breeding.Item IPR 106: new Arabica coffee cultivar, resistant to some Meloidogyne paranaensis and M. incognita nematode populations of Paraná(Crop Breeding and Applied Biotechnology, 2020) Sera, Gustavo Hiroshi; Machado, Andressa Cristina Zamboni; Ito, Dhalton Shiguer; Shigueoka, Luciana Harumi; Silva, Santino Aleandro da; Sera, TumoruCultivar IPR 106 resulted from a spontaneous hybridization between “Icatu IAC 925” and an unknow dwarf plant. It is a dwarf cultivar with high rusticity, late ripening cycle, large grains, excellent cup quality and resistance to some populations of the nematodes Meloidogyne paranaensis and M. incognita found in the state of Paraná.Item Associação de Pseudomonas syringae pv. garcae com algumas características agronômicas em cafeeiros F2 segregantes para o gene erecta(Editora UFLA, 2004-09) Sera, Gustavo Hiroshi; Sera, Tumoru; Altéia, Marcos Zorzenom; Androcioli Filho, Armando; Azevedo, José Alves de; Petek, Marcos Rafael; Ito, Dhalton ShiguerA bacteriose causada pela Pseudomonas syringae pv. garcae provoca grandes prejuízos em algumas regiões da cafei- cultura brasileira, como Paraná, São Paulo e Minas Gerais, principalmente em lavouras novas, podadas e expostas ao vento. Com este trabalho, objetivou-se estudar nas plantas F 2 do cruzamento entre os genótipos IAPAR-59 e Catuaí Erecta a associação entre as características vigor vegetativo e ângulos de inserção das ramificações plagiotrópicas sobre a intensidade de ocorrência da bacteriose. Avaliaram-se, em outubro de 2001, 316 plantas F 2 plantadas no IAPAR de Londrina em outubro de 1998. A escala de notas de avaliação da incidência de bacteriose adotada foi de 1 a 5, sendo 1 = plantas sem lesão e 5 = plantas com muitas lesões. As notas de vigor vegetativo adotadas foram de 1 a 5, sendo 1 para folhas de coloração verde-claras e 5 para folhas verde- escuras. Para a inserção dos ramos plagiotrópicos sobre os ortotrópicos, as notas variaram de 1 a 3, sendo 1 = normal (45 a 70o), 2 = semi-ereta (25 a 40o) e 3 = ereta (5 a 20o). Estimou-se o coeficiente de correlação de Pearson para verificar a associação entre as características vigor vegetativo e do ângulo de inserção das ramificações plagiotrópicas com a intensidade de ocorrência da bacteriose nos cafeeiros. A correlação estimada entre a intensidade da bacteriose com o ângulo de inserção dos ramos plagiotrópicos foi de r = + 0,2087**. Não houve correlação significativa entre a bacteriose e o vigor vegetativo. Assim, plantas com ramificação plagiotrópica ereta são predispostas a uma maior incidência de bacteriose.Item Genetic diversity among forty coffee varieties assessed by rapd markers associated with restriction digestion(Instituto de Tecnologia do Paraná - Tecpar, 2005-07) Diniz, Leandro Eugênio Cardamoni; Ruas, Claudete de Fátima; Carvalho, Valdemar de Paula; Torres, Fabrício Medeiros; Ruas, Eduardo Augusto; Santos, Melissa de Oliveira; Sera, Tumoru; Ruas, Paulo MaurícioThe genetic variability of 40 accessions of C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among C. arabica genotypes.Item Partial resistance to fruit necrosis associated to Colletotrichum spp. among arabic coffee genotypes(Instituto de Tecnologia do Paraná - Tecpar, 2007-05) Sera, Gustavo Hiroshi; Sera, Tumoru; Ito, Dhalton Shiguer; Azevedo, José Alves de; Ribeiro Filho, Claudionor; Mata, João Siqueira daThe aim of this study was to evaluate the resistance to fruit necrosis associated to Colletotrichum spp. in Coffea arabica L.. Twenty-two arabic coffee genotypes were performed in a randomized block design, with three replications and ten plants per plot. They were evaluated for the variables fruit necrosis, luminosity index inside of the plant canopy and fruits per productive nodes of the plants. There was genetic variability for the resistance to fruit necrosis. Different partial resistance levels, varying from highly susceptible (score = 3.89) to moderately resistant (score = 1.77) were observed. The genotypes that presented moderate resistance were the cultivars IPR- 100, IPR-103, IPR-105 and IPR-108 and the coffee selection IAPAR-00023.Item Resistance to bacterial halo blight in Arabica coffee lines derivative from the genotype C1195-5-6-2 under natural infection conditions(Crop Breeding and Applied Biotechnology, 2018-01) Andreazi, Elder; Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Carducci, Fernando Cesar; Shigueoka, Luciana Harumi; Santos, Willian Gabriel dosThe aim of this study was to identify resistance to bacterial halo blight in Arabica coffee lines carrying Coffea racemosa genes. Eighteen Arabica coffee lines derivative from the genotype C1195-5-6-2, and the cultivars IAPAR 59 and IPR 99 were evaluated for resistance to bacterial halo blight in two trials carried out in field conditions, in Londrina, PR, Brazil. The cultivars Mundo Novo IAC 376-4 and Catuaí Vermelho IAC 81 were included as susceptible controls. Ten lines and the cultivar IAPAR 59 showed resistance to bacterial halo blight. The cultivar IPR 99 presented intermediate reaction, and the controls were very susceptible. This is the first study to show that lines derivative from the genotype C1195-5-6-2, which has C. racemosa genes, could be a source of resistance to bacterial halo blight in coffee breeding programs.Item IPR 100 – Rustic dwarf Arabica coffee cultivar with resistance to nematodes Meloidogyne paranaensis and M. incognita(Crop Breeding and Applied Biotechnology, 2017-03) Sera, Tumoru; Sera, Gustavo Hiroshi; Fazuoli, Luiz Carlos; Machado, Andressa Cristina Zamboni; Ito, Dhalton Shiguer; Shigueoka, Luciana Harumi‘IPR 100’ was derived from a crossing between “Catuaí” and a hybrid(“Catuaí” x BA-10 coffee). ‘IPR 100’ is a medium size dwarf cultivar with high rusticity, with late ripening cycle, and with resistance to Meloidogyne paranaensis and to some M. incognita populations.Item Selection of Arabic coffee progenies with rust resistance(Crop Breeding and Applied Biotechnology, 2014-06) Shigueoka, Luciana Harumi; Sera, Gustavo Hiroshi; Sera, Tumoru; Fonseca, Inês Cristina de Batista; Mariucci Junior, ValdirThe aim of this study was to select high-yielding coffee progenies with resistance to coffee leaf rust for the State of Paraná (Brazil). Field experiments in a randomized block design were carried out in Itaguajé and Congonhinhas. Yield, vegetative vigor and rust resistance of nine progenies of Arabic coffee and three check cultivars were evaluated. Many genotypes derived from “Sarchi- mor” and “Catucaí” were susceptible. Three coffee genotypes of Sarchimor germplasm and the F 6 generation of genotype “Catuaí x (Catuaí x BA-10 coffee)” were selected to advance generations and are promising to become new cultivars for being higher-yielding than ‘IAPAR 59’ and ‘Tupi IAC 1669-33’ and having a large number of plants with complete rust resistance.Item IPR 107 – Dwarf arabic coffee cultivar with resistance to coffee leaf rust(Crop Breeding and Applied Biotechnology, 2013-10) Sera, Tumoru; Sera, Gustavo Hiroshi‘IPR 107’ was derived from a cross between ‘IAPAR 59’ and ‘Mundo Novo IAC 376-4’. ‘IPR 107’ is a dwarf medium size plant with medium precocity in ripening and with complete resistance to rust races in this time. This cultivar presents superior quality and high yield in many coffee regions.